▎ 摘　　要

NOVELTY - Constructing nano-graphene composite material carrying antibody-chemokine (C-C motif) ligand 2 (CCL2) antibody comprises e.g. using poly(maleic anhydride-alt-1-octadecene) (C18PMH), methoxy polyethylene glycol amine (mPEG-NH2) and amine-polyethylene glycol-Boc (NH2-PEG-BOC), synthesizing C18-PMH-PEG-NH2 nano composites containing amino functional groups on surface, synthesizing of reduced graphene oxide-iron oxide nanoparticles-polyethylene glycol-amine reduced graphene oxide-iron oxide nanoparticles (RGO-IONP-PEG-NH2 RGO-IONP) and C18-PMH-PEG-NH2 nanocomposite, ultrasonically mixing, removing excess C18-PMH-PEG-NH2 nanocomposite to obtain RGO-IONP-PEG-NH2 nanocomposite with amino functional groups on the surface and synthesizing RGO-IONP-PEG-CCL2 nano-functional complex, adding CCL2 antibody and RGO-IONP-PEG-NH2 nano-complex to a microwave tube, ultrasonically protecting from light and using combination of nano metal oxide and 2-chlorophenylboronic acid as a catalyst. USE - The method is useful for constructing nano-graphene composite material carrying CCL2 antibody. ADVANTAGE - The method improves the yield of RGO-IONP-PEG-CCL2 nano-functional composite, has low reaction temperature and mild reaction conditions and realizes a high load rate of nano-graphene composite material to CCL2 antibody. DETAILED DESCRIPTION - Constructing nano-graphene composite material carrying antibody-chemokine (C-C motif) ligand 2 (CCL2) antibody comprises (1) using poly(maleic anhydride-alt-1-octadecene) (C18PMH), methoxy polyethylene glycol amine (mPEG-NH2) and NH2-PEG-BOC, synthesizing C18-PMH-PEG-NH2 nano composites containing amino functional groups on surface, (2) synthesizing of reduced graphene oxide-iron oxide nanoparticles-polyethylene glycol-amine reduced graphene oxide-iron oxide nanoparticles (RGO-IONP-PEG-NH2 RGO-IONP) and C18-PMH-PEG-NH2 nanocomposite, ultrasonically mixing at a mass ratio of 1:10-15 for 80-90 minutes, removing excess C18-PMH-PEG-NH2 nanocomposite to obtain RGO-IONP-PEG-NH2 nanocomposite with amino functional groups on the surface and (3) synthesizing RGO-IONP-PEG-CCL2 nano-functional complex, adding CCL2 antibody and RGO-IONP-PEG-NH2 nano-complex to a microwave tube according to a mass ratio of 1:8-9, ultrasonically protecting from light for 20-25 minutes, using combination of nano metal oxide and 2-chlorophenylboronic acid as a catalyst, placing it under microwave radiation, catalyzing the reaction, where the temperature of the microwave is less than or equal to 50 degrees C, and the microwave time is 15-20 minutes, promoting the connection of CCL2 antibody and RGO-IONP-PEG-NH2 nanocomposite through amidation reaction, centrifuging the reaction solution at 6000-8000 revolutions/minute for 2-3 minutes, removing unstable particles or aggregates, and collecting the supernatant, using 100-110 KD ultrafiltration tube and removing the unconnected CCL2 antibody to obtain RGO-IONP-PEG-CCL2 nano-functional complex.