▎ 摘　　要

NOVELTY - Preparing hyaluronic acid/arginine-glycine-aspartic acid-modified graphene oxide targeting medicinal composite material involves dispersing graphene oxide (GO) in deionized water to form dispersion liquid. Graphene oxide dispersion liquid is subjected to amination and oxidation by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) activating agent, N-hydroxysuccinimide (NHS)-mediated carboxyl amine reaction to obtain GO-ADH. N-(3-Aminopropyl)methacrylamide hydrochloride is covalently connected with HA with amide bond, stirred for 4-6 hours, and carried out dialyzing. USE - Method for preparing hyaluronic acid/arginine-glycine-aspartic acid-modified graphene oxide targeting medicinal composite material (claimed). ADVANTAGE - The method is simple to operate, enables to prepare hyaluronic acid/arginine-glycine-aspartic acid-modified graphene oxide targeting medicinal composite material, ensures mild experiment condition is mild, high loading of whole carrier system and long-acting slow release effect, is pH sensitive, has high release rate at low pH value environment and important clinical significance, and is suitable for tumor tissue microenvironment. DETAILED DESCRIPTION - Preparing hyaluronic acid/arginine-glycine-aspartic acid-modified graphene oxide targeting medicinal composite material involves dispersing graphene oxide (GO) in deionized water to form dispersion liquid. Graphene oxide dispersion liquid is subjected to amination and oxidation by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) activating agent, N-hydroxysuccinimide (NHS)-mediated carboxyl amine reaction to obtain GO-ADH. N-(3-Aminopropyl)methacrylamide hydrochloride is covalently connected with HA with amide bond, stirred for 4-6 hours, and carried out dialyzing, freezing and drying to obtain HA-METH. HA-METH is dispersed in phosphoric acid buffer liquid and GO-ADH is added at high temperature under stirring, washed it and dried to obtain GO-HA-METH. GO-HA-METH solution is mixed in deionized water and EDC and NHS are added to regulate pH at 5-8, and mercapto and arginine-glycine-aspartic acid (RGD) are added. Mixture is centrifuged for 24 hours, washed and dried to obtain GO-HA-RGD composite material. GO-HA-RGD composite material is mixed in deionized water, and melphalan (MEL) and dimethyl sulfoxide (DMSO) solution are added slowly dropwise and stirred for 24 hours. Adriamycin (DOX) and DMSO solution are added in obtained product slowly dropwise and triethylamine is added with dropwise to regulate pH at weak alkaline and stirred for 24 hours in light-proof at room temperature. Product is frozen, washed and dried. An INDEPENDENT CLAIM is included for a method for preparing graphene oxide powder, which involves: (A) mixing graphite powder uniformly with solid sodium nitrate under ice bath condition; (B) adding concentrated sulfuric acid and solid potassium permanganate in mixture and stirring at 0-5 degrees C for 1-2 hours; (C) stirring the product at 98 degrees C for 0.5 hours, adding deionized water and hydrogen peroxide to carry out termination reaction; (D) filtering and washing the product and drying to obtain solid powder; (E) dispersing solid powder into deionized water, ultrasonic peeling at 32-38 degrees C to obtain graphene oxide nanosheets; (F) centrifuging graphene oxide nanosheets to obtain brown GO suspension; and (G) freezing and drying GO powder, where graphite powder, sodium nitrate and potassium permanganate mass ratios are 1:0.5:(3-5).