▎ 摘　　要

NOVELTY - Method of preparing dual drug fluorescent magnetic microsphere, involves synthesizing graphene quantum dot, adding paclitaxel and graphene quantum dots to the mixture, adjusting the pH, stirring, centrifuging the solution, drying the precipitate, adding kaempferol magnetic microspheres hydroxysuccinimide to the solution, washing the reacted solution, drying, preparing double fluorescent magnetic drug-loaded microspheres by drug delivery experiments, culturing double fluorescent magnetic drug-loaded microspheres with Hela cells, and detecting the microspheres. USE - The method is useful for preparing dual drug fluorescent magnetic microsphere (claimed). ADVANTAGE - The prepared fluorescent magnetic microsphere reduces toxic or side effects. DETAILED DESCRIPTION - Method of preparing dual drug fluorescent magnetic microsphere, involves (a) mixing hydrophobic drug of kaempferol in oil-modified iron oxide magnetic fluid, adding bovine serum albumin solution to the mixture, stirring the mixture to obtain uniform emulsion, crushing the emulsion at 250-400 W for 10-20 minutes, drying the crushed mixture, where the modified oil-based magnetic fluid of the magnetic fluid medium is cyclohexane, the mass fraction of the iron oxide is 20%, the mass fraction of the bovine serum albumin solution is 2%, and the mass ratio of the hydrophobic kaempferol and iron oxide is 1:400, (b) synthesizing graphene quantum dot with particle size of 5-10 nm, adding paclitaxel and graphene quantum dots to the mixture, adjusting the pH of the solution using phosphate buffer solution, uniformly stirring the mixture for 12-24 hours, centrifuging the solution, drying the precipitate, where the mass volume ratio of the paclitaxel and phosphate buffer solution is 1 mg:30 ml, the volume ratio of the graphene quantum dots and phosphate buffer solution is 15 mg:30 ml, (c) adding kaempferol magnetic microspheres hydroxysuccinimide to the solution, where the mass concentration of the kaempferol is 0.4-0.6 mg/ml, reacting the mixture at 20-30 degrees C for 0.5-1 hours, washing the reacted solution, and drying the solution at 30-50 degrees C, (d) preparing double fluorescent magnetic drug-loaded microspheres by drug delivery experiments, and (e) culturing double fluorescent magnetic drug-loaded microspheres with Hela cells, and detecting the microspheres by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity.