▎ 摘　　要

NOVELTY - Aptamer, is new. The nucleotide sequences of the aptamers comprises fully defined sequence of 97 base pairs (SEQ ID No. 1) as given in the specification, and fully defined sequence of 97 base pairs (SEQ ID No.2) as given in the specification. USE - The aptamer/single stranded DNA library is useful in kit for screening aptamer for specifically recognizing aflatoxin M1 (all claimed) in food. ADVANTAGE - The aptamer/single stranded DNA library: are accurate, fast, sensitive detection of aflatoxin M1 in food has wide application prospect. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: a method for screening a single stranded DNA library for specific recognition of the aptamer of aflatoxin M1, comprising the single-stranded DNA library is on the basis of the sequence comprises sequence of 5'--3' (SEQ ID No: not defined), sequence of 5'--3' (SEQ ID No: not defined), the sequence segment capable of forming stem-ring structure is inserted into the random area of N51, the five-segment initial ssDNA library is composed of the sequence segment 5' the stem-loop structure, and the N51 represents a sequence formed by ligating 51 arbitrary nucleotide bases;cctctctatgggcagtcggtgatggagaatgaggaacccagtgcag a screening method of aptamer for specifically identifying aflatoxin M1, comprises (1) adding aflatoxin M1 random single-stranded DNA library and target incubation to the random single-stranded DNA library, mixing incubation, the random single-stranded DNA library and aflatoxin M1 fully combined to form incubation mixed solution, mixing graphene oxide with the incubation mixed solution, after incubation, solid-liquid separating and taking supernatant, and obtaining ssDNA of obtain with target, (2) PCR amplifying the ssDNA obtained in the step (1) as the template to carry out PCR amplification, (3) preparing the amplified product in the step (2) into single chain DNA to obtain the next screening library, (4) replacing multi-wheel screening the random single-stranded DNA library in step (1) by the next round screening library in step (3), repeating multi-wheel screening according to step (1)-(3), (5) high throughput sequencing screening, the screening library obtained in the last round screening in the step (3) for high throughput sequencing analysis, detecting the affinity and specificity of the obtained sequence and aflatoxin M1, obtaining nucleic acid aptamer for specifically identifying aflatoxin M1; and a kit, comprising the aptamer.