▎ 摘　　要

NOVELTY - Preparing resistor immunochromatographic sensor comprises (i) blending graphene and carboxymethyl cellulose in a ratio of 2.5:0.5 to make viscous graphene powder, adding specific amount of isopropanol aqueous solution to make the volume reach 50 ml, stirring at room temperature for 30 minutes to make it stable into a graphene slurry, slowly pouring the graphene slurry onto the customized screen for screen printing, printing on the nitrocellulose (NC) film to become a patterned conductive coating, and preparing the patterned conductive NC film by air-drying in well-ventilated place at room temperature, (ii) taking specific amount of polyaniline (PANI) in phosphate-buffered saline buffer solution containing 10% dimethylformamide and 1% lithium chloride to make 1 mg/ml polyaniline dispersion solution, the phosphate-buffered saline buffer solution is 10 mM, pH is 7.6, adding 80 mu l antibody solution with concentration of 150 mu g/ml to the polyaniline dispersion. USE - The method is useful for preparing resistor immunochromatographic sensor. ADVANTAGE - The sensor: compared with the prior art, discards the non-immune response process introduced in common quantitative immunochromatographic methods and the signal basis generated by non-immune response components; realizes direct electrical testing; flexibly applies to precision device measurement and small device testing according to requirements; and has both accurate detection and portable quantitative functions. DETAILED DESCRIPTION - Preparing resistor immunochromatographic sensor comprises (i) blending graphene and carboxymethyl cellulose in a ratio of 2.5:0.5 to make viscous graphene powder, adding specific amount of isopropanol aqueous solution to make the volume reach 50 ml, stirring at room temperature for 30 minutes to make it stable into a graphene slurry, slowly pouring the graphene slurry onto the customized screen for screen printing, printing on the nitrocellulose (NC) film to become a patterned conductive coating, and preparing the patterned conductive NC film by air-drying in well-ventilated place at room temperature, (ii) taking specific amount of polyaniline (PANI) in phosphate-buffered saline buffer solution containing 10% dimethylformamide and 1% lithium chloride to make 1 mg/ml polyaniline dispersion solution, the phosphate-buffered saline buffer solution is 10 mM, pH is 7.6, adding 80 mu l antibody solution with concentration of 150 mu g/ml to the polyaniline dispersion, reacting at room temperature for 30 minutes to prepare polyaniline-antibody mixed solution, adding 0.1 ml trimethylolaminomethane buffer solution containing 0.1% bovine serum albumin (BSA) to the polyaniline-antibody mixture, the trimethylolaminomethane buffer solution is 10 mM, pH is 7.6, and reacting at room temperature for 30 minutes, using the trisbuffer as detergent to perform centrifugal washing, centrifugally washing for 3 minutes at speed of 13000 revolutions/minute, repeating for three times, where the retained centrifugal pellet is the polyaniline-antibody complex, dispersing the polyaniline-antibody complex in 1 ml phosphate-buffered saline buffer, and storing in a refrigerator at 4 degrees C, (iii) assembling the bottom plate, conductive NC membrane, immune binding pad, water absorption pad, and sample pad from bottom to top in order, and (iv) loading the sample pad of the resistance immunochromatographic sensor with the sample solution to complete the chromatographic process, and air-drying or drying at 60 degrees C to remove the excess aqueous phase medium, where the wires of the detection device are respectively connected to graphene-polyaniline-graphene to form an instantaneous current loop to detect the resistance signal of the polyaniline immune binding band.