▎ 摘　　要

NOVELTY - High-throughput liquid phase suspension type biological chip comprises a suspension medium and multifunctional microspheres uniformly dispersed in the suspension medium; the multifunctional microsphere comprises inorganic/polymer composite micro-sphere and biological probe coated on the surface of the inorganic/polymer composite microspheres. The inorganic/polymer microspheres include graphene nanosheets, graphene quantum dot shells dispersed on the surface of the graphene nanosheets and modified on the surface of the graphene quantum dot shells. USE - High-throughput liquid phase suspension type biological chip is used for virus detection. ADVANTAGE - The chip has good monodispersity and high sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparation method of high-throughput liquid phase suspension biochip comprising: (1) mixing graphene quantum dot solution and graphene nano-sheet dispersion and performing ultrasonic treatment for 30-120 minutes at 500-1000 W to obtain composite dispersion; (2) mixing and stirring the microcrystalline cellulose and sulfuric acid solution uniformly, heating to 40degrees Celsius to react for 1-2 hours at a rate of 1degrees Celsius/minute, after finishing the reaction, adding deionized water for reaction, centrifuging to obtain the reaction liquid, freezing the dry dispersion solution at -10 to -20degrees Celsius for 10-20 hours to obtain the nano cellulose crystal; (3) dispersing the prepared nano-cellulose crystal in the deionized water, then adding (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl or (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl (TEMPO), sodium bromide, stirring and dissolving, adding sodium hypochlorite solution, and adding alkali to adjust the pH to 10-11, after the reaction until the pH is not changed, dripping ethanol to stop the reaction, centrifuging and collecting the supernatant, freezing the dry at -10 to -20degrees Celsius for 10-24 hours to obtain carboxylated cellulose nanocrystals; (4) adding the carboxylated nano cellulose crystal into the composite dispersion, continuously adding acrylamide, N,N-methylene-bisacrylamide, heating to 70-80degrees Celsius, adding potassium persulfate, stirring and reacting for 2-5 hours, after finishing reaction, cooling to room temperature, freezing and drying the reaction liquid, to obtain multifunction microsphere; (5) dispersing the multifunctional microsphere to deionized water dispersion liquid, then adding mixed solution of sodium dihydrogen phosphate and 5 mol/l sodium hydroxide solution to stir and disperse, then adding phosphate buffered saline (PBS) solution with concentration of 50 mg/ml ethylene dichloride (EDC) and 50 mg/ml sulfo-N-hydroxysuccinimide (S-NHS), continuously stirring and dispersing treatment, adding probe, 10degrees Celsius, 500rpm vibration treatment and incubation under the shading condition for 10-12 hours, after finishing using cell washing liquid to wash to remove the micro-coated probe, adding cell confining liquid, shading sealing reaction for 20-30 minutes to obtain the chip.