▎ 摘　　要

NOVELTY - Hybrid graphene electrode-based electrochemical biosensor characterized in that flow of electrons is generated through graphene metal composite comprises enzymes that drive biochemical reactions, a reactant reacting with the enzyme, resultant substances generated by the enzymatic reaction, and a measuring electrode for detecting the enzymatic reaction product, where the measuring electrode comprises a graphene metal composite in which several micro metal particles and a part of the graphene composite layer are connected, the fine metal particles are bound to the surface or inside of the graphene composite layer, and some of the fine metal particles are mutually bonded and solidified, the graphene composite layer has a three-dimensional structure in which several layers of graphene are stacked and bent in an arbitrary direction, and a portion of the empty space between the fine metal particles is a porous structure filled with the graphene composite layer and connected to each other. USE - The hybrid graphene electrode-based electrochemical biosensor is used for detecting hybrid graphene electrode-based specific antigen, preferably Alzheimer's disease factor (all claimed). ADVANTAGE - The hybrid graphene electrode-based electrochemical biosensor has high electrical conductivity due to structural feature, selectivity for low-concentration antigens, and excellent specificity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for detecting hybrid graphene electrode-based specific antigen, which involves separating plasma from blood, binding the disease factor antigen contained in the separated plasma to an enzyme-linked secondary antibody, moving the secondary antibody bound to the antigen to a reaction chamber, binding the transferred antigen to the primary antibody immobilized on the inner surface of the reaction chamber, washing the inner surface of the reaction chamber with a cleaning solution, moving a solution containing an enzyme substrate into a chamber to react with an enzyme linked to the secondary antibody, and sensing a current signal generated by reaction between the enzyme substrate and the enzyme using the hybrid graphene electrode-based electrochemical biosensor.