• 专利标题:   Preparing near-infrared fluorescent probe for selectively recognizing glutathione, involves subjecting pyrene to nitric acid reflux and hydrothermal reaction to obtain stock solution of aminated graphene quantum dots, filtering, dialyzing, and freeze drying.
  • 专利号:   CN115948163-A
  • 发明人:   CHEN J, CHEN H, WANG Y, YIN H, ZHAO L, WU M, XIE J
  • 专利权人:   YANGZHOU POLYTECHNIC INST
  • 国际专利分类:   B82Y020/00, B82Y040/00, C09K011/02, C09K011/08, C09K011/65, G01N021/64
  • 专利详细信息:   CN115948163-A 11 Apr 2023 C09K-011/65 202338 Chinese
  • 申请详细信息:   CN115948163-A CN10001528 03 Jan 2023
  • 优先权号:   CN10001528

▎ 摘  要

NOVELTY - Preparing near-infrared fluorescent probe involves using pyrene as a raw material, and subjecting to nitric acid reflux and hydrothermal reaction to obtain a stock solution of aminated graphene quantum dots. The stock solution is filtered, dialyzed, and freeze-dried to obtain solid aminated graphene quantum dots. The aminated graphene quantum dots and p-cyanobenzoic acid are reacted in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide for 20 minutes. The resultant is dialyzed in the dark for 2 days, and then freeze-dried in the dark to finally obtain the p-cyanobenzoic acid-graphene quantum dot composite material. USE - Method for preparing near-infrared fluorescent probe for selectively recognizing glutathione. ADVANTAGE - The aminated graphene quantum dots can be well combined with p-cyanobenzoic acid through chemical bonds, helps in exhibiting excellent photoelectric properties, has low toxicity and good biocompatibility, and acts as an excellent living fluorescent probe. The sulfhydryl group on glutathione attacks the cyano group in the composite nanomaterial, resulting in fluorescence quenching, for realizing the fluorescence detection near glutathione.