▎ 摘 要
NOVELTY - Preparing near-infrared fluorescent probe involves using pyrene as a raw material, and subjecting to nitric acid reflux and hydrothermal reaction to obtain a stock solution of aminated graphene quantum dots. The stock solution is filtered, dialyzed, and freeze-dried to obtain solid aminated graphene quantum dots. The aminated graphene quantum dots and p-cyanobenzoic acid are reacted in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide for 20 minutes. The resultant is dialyzed in the dark for 2 days, and then freeze-dried in the dark to finally obtain the p-cyanobenzoic acid-graphene quantum dot composite material. USE - Method for preparing near-infrared fluorescent probe for selectively recognizing glutathione. ADVANTAGE - The aminated graphene quantum dots can be well combined with p-cyanobenzoic acid through chemical bonds, helps in exhibiting excellent photoelectric properties, has low toxicity and good biocompatibility, and acts as an excellent living fluorescent probe. The sulfhydryl group on glutathione attacks the cyano group in the composite nanomaterial, resulting in fluorescence quenching, for realizing the fluorescence detection near glutathione.