▎ 摘 要
NOVELTY - Kit comprises upstream and downstream primer sequences of miRNA. The upstream and downstream primer sequences and auxiliary components of U6, miRNA comprises miRNA934, miRNA4444-2, miRNA3655, miRNA1301 molecules, the miRNAs in isolated samples of pancreatic cancer patients treated with the kit and prognosis to obtain the expression of the miRNA combination in the sample, The prognosis of patients with pancreatic cancer was determined according to the high and low expression components. USE - The kit is useful for detecting pancreatic cancer prognosis condition. ADVANTAGE - The invention detects the expression condition of 4 miRNAs specifically expressed in the patient sample, and establishes a diagnosis reagent box distinguishing patient cancer prognosis bad, which can comprehensively improve the pancreatic cancer prognosis curative effect and metastasis, and the accuracy of recurrence prediction.