▎ 摘　　要

NOVELTY - Purifying nucleic acids from a sample containing nucleic acids to be purified, comprises (i) contacting the sample with a solid phase material having a glass surface in presence of a binding buffer containing a chaotropic salt to bind the nucleic acids to the glass surface and obtain a solid phase material having the nucleic acids bound to its glass surface, (ii) washing the solid phase material having the nucleic acids bound to its glass surface with a first wash buffer containing a 2-4C aliphatic alcohol, (iii) washing the solid phase material having the nucleic acids bound to its glass surface with a final wash buffer comprising at least 50 wt.% dimethyl sulfoxide, and (iv) eluting the nucleic acids from the glass surface with an elution buffer to provide purified nucleic acids. USE - The method is useful for purifying nucleic acids from a sample containing nucleic acids to be purified, where the purified nucleic acids are used for reverse transcription PCR, and/or isothermal amplification. The buffer containing at least 50 wt.% dimethyl sulfoxide is useful for purifying nucleic acids (all claimed). ADVANTAGE - The method utilizes small magnetic particles which offers good combination of surface to volume ratio and high magnetic moment per particle. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a kit for purifying nucleic acids from a sample containing nucleic acids to be purified comprising the solid phase material having a glass surface, the binding buffer, an aqueous buffer for preparing the first wash buffer, the final wash buffer, and optionally the elution buffer.