• 专利标题:   Method for preparing graphene-based hemoglobin sensor electrode for detecting hemoglobin in feces, involves preparing graphene modified electrode, applying dispersed droplets onto electrode followed by promoting electrochemical reaction.
  • 专利号:   CN108241012-A
  • 发明人:   YING Z, ZHANG R, YANG W, HAO Y, SUN B, SONG C, LI J, ZENG Y, CHENG H
  • 专利权人:   INST METAL RES CHINESE ACAD SCI, LIAONING CANCER HOSPITAL INST
  • 国际专利分类:   G01N027/327
  • 专利详细信息:   CN108241012-A 03 Jul 2018 G01N-027/327 201858 Pages: 10 Chinese
  • 申请详细信息:   CN108241012-A CN11225437 27 Dec 2016
  • 优先权号:   CN11225437

▎ 摘  要

NOVELTY - A graphene-based hemoglobin sensor electrode preparing method involves preparing a graphene modified electrode. The graphene oxide prepared by the chemical oxidation method is dispersed in a phosphate buffer solution to form a stable dispersion liquid, and the dispersed droplets are applied onto the cleaned glassy carbon electrode, and the graphene oxide-modified electrode is dried at room temperature. Reduction of graphene oxide is performed on the modified electrode. The modified electrode is utilized as a working electrode in a phosphate buffer solution having molar concentration of 0.05-0.2 M, and three-electrode system is formed with a saturated calomel reference electrode and platinum plate counter electrode followed by performing cyclic voltammetry scanning at -1.5 to 0 voltage that reduces graphene oxide to graphene, improving conductivity of electrode and the adsorption performance of hemoglobin, and promoting direct electrochemical reaction between hemoglobin and electrode. USE - Method for preparing graphene-based hemoglobin sensor electrode that is utilized for detecting hemoglobin in feces, and urine (claimed), and screening fecal occult blood in patients with suspected gastrointestinal malignancies. ADVANTAGE - The biosensor has high sensitivity, better stability, wide linear range and strong anti-interference ability. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a for use of graphene-based hemoglobin sensor electrode for detecting hemoglobin in feces, and urine.