▎ 摘　　要

NOVELTY - Fluorescent biosensor, obtained by comprising dispersing molybdenum disulfide (MoS2) powder in N,N-dimethylformamide solution to obtain the molybdenum disulfide dispersion, dispersing graphene oxide powder in pure water obtain the graphene oxide dispersion, adding ascorbic acid, and mixing to obtain reduced graphene oxide (RGO) dispersion liquid, mixing MoS2dispersion liquid and RGO dispersion liquid, obtaining molybdenum disulfide-reduced graphene oxide (MoS2-RGO) solution, centrifuging, and washing and drying the separated solid and drying to obtain MoS2-RGO, stirring and stirring and mixing the MoS2-RGO solid with pure water to obtain the MoS2-RGO dispersion, and mixing the MoS2-RGO solution and the N,S-GQDs-GP73Apt solution, and incubating to quench the fluorescence of the nitrogen-sulfur-doped graphene quantum dots (N,S-GQDs) to form N,S-GQDsGP73Apt/MoS2-RGO Fluorescence resonance energy transfer (FRET) fluorescent biosensors. USE - The biosensor is useful for Golgi protein-73 detection based on fluorescence resonance energy transfer. ADVANTAGE - The method simplifies the whole detection process according to the one-step reaction principle, the detection period is short, the cost is low, and the experiment is strong. DETAILED DESCRIPTION - Fluorescent biosensor comprising (1a) preparing nitrogen and sulfur-doped graphene quantum dots N, taking citric acid and thiourea, adding pure water to constant volume, stirring evenly, heating, and cooling, adding ethanol and stirring, and dialyzing with a dialysis bag and freeze drying to obtain nitrogen-sulfur-doped graphene quantum dots (N,S-GQDs) solid, and (1b) measuring N,S-GQDs and GP73 aptamer GP73Apt, using a cross-linking agent to activate cross-linking, and incubating to obtain N,S-GQDs-GP73Apt solution, (2a) dispersing molybdenum disulfide (MoS2) powder in N,N-dimethylformamide solution to obtain the molybdenum disulfide dispersion, (2b) dispersing graphene oxide powder in pure water obtain the GO dispersion, adding ascorbic acid, and mixing to obtain reduced graphene oxide (RGO) dispersion liquid, (2c) mixing MoS2 dispersion liquid and RGO dispersion liquid, obtaining molybdenum disulfide-reduced graphene oxide (MoS2-RGO) solution, centrifuging, and washing and drying the separated solid and drying to obtain MoS2-RGO, stirring and stirring and mixing the MoS2-RGO solid with pure water to obtain the MoS2-RGO dispersion, and (2d) mixing the MoS2-RGO solution and the N,S-GQDs-GP73Apt solution, and incubating to quench the fluorescence of the N,S-GQDs to form N,S-GQDsGP73Apt/MoS2-RGO Fluorescence resonance energy transfer (FRET) fluorescent biosensors, scanning with a fluorescence spectrophotometer, fixing the excitation wavelength at 368 nm, measuring the fluorescence intensity at 450 nm, and recording as F0, (3) (3a) adding different concentrations of GP73 solution to N,S-GQDsGP73Apt/MoS2-RGO FRET fluorescent biosensor, incubating, detecting the peak change at 450 nm, scanning with a fluorescence spectrophotometer, fixing the excitation wavelength at 368 nm, measuring the fluorescence intensity at 450 nm, denoted as F1, and (3b) calculating the minimum detection limit of the fluorescent biosensor, and repeating the above method with actual sample.