▎ 摘　　要

NOVELTY - Fluorescent colorimetric nucleic acid aptamer sensor comprises platinum/gold/rhodium nanoparticles, metal mesh-graphene oxide and fluorescent group modified profenofos nucleic acid aptamer, where fluorophore-modified profenofos nucleic acid aptamer is non-specifically adsorbed on the platinum/gold/rhodium nanoparticles, and non-specifically adsorbed with the metal mesh-graphene oxide to form an assembly, which is the dual detection profenofos fluorescence colorimetric nucleic acid aptamer sensor. USE - Fluorescent colorimetric nucleic acid aptamer sensor for preparing detection reagent for dual detection of profenofos pesticide (claimed). ADVANTAGE - The sensor has simple structure, low detection cost, fast detection, high detection sensitivity, strong specificity and low requirement to the detection instrument, and does not need to perform complex pre-treatment to the sample to be detected. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following: (1) a method for preparing a fluorescent colorimetric nucleic acid aptamer sensor, which involves centrifuging the platinum/gold/rhodium nanoparticles (PtAuRh NPs) solution at 8000 rpm for 10 minutes, discarding the supernatant, and resuspend the pellet in phosphate-buffered saline (PBS), adding the fluorophore-modified profenofos nucleic acid aptamer, and shake overnight at 37 degrees C, centrifuging the above mixture at 8000 rpm for 3 times for 10 minutes, washing the precipitate with alcohol to obtain PtAuRh NPs-Aptamer, which is redispersed in PBS, immersing metal mesh-graphene oxide in the PBS solution of PtAuRh NPs-Aptamer for 15 minutes after the reaction is completed, the PtAuRh NPs-Aptamer is connected to the metal mesh-graphene oxide to obtain a fluorescent colorimetric nucleic acid aptamer sensor; and (2) a method for detecting profenofos using the sensor, which involves adding the fluorescent colorimetric nucleic acid aptamer sensor for double detection of profenofos pesticide into the solution to be detected, mixing and incubating at room temperature for 15 minutes, removing the metal mesh-graphene oxide, adding Hydrogen peroxide, 3,3`,5,5`-tetramethylbenzidine, and sodium acetate- acetic acid buffer to the remaining solution in sequence after reacting for 3 minutes at room temperature, quickly recording the absorbance signal of the resulting solution at 652 nm or detect the fluorescence intensity of the fluorophore by UV-visible spectroscopy, and comparing the standard curve to obtain concentration of profenofos.