▎ 摘　　要

NOVELTY - Presenting an antimicrobial activity of Titanium dioxide (TiO2) and Zinc oxide (ZnO)/Graphene oxide (GO) nanomaterial on Enterococcus faecalisMTCC 439 involves (a) preparing the inoculum using 24 hours plate cultures of the test bacteria (Enterococcus faecalisMTCC 439); (b) suspending the colonies in 0.85% saline; (c) loading the suspension on a sterile cotton swab that is rotated multiple times and pressed firmly against the inside wall of the tube to remove excess inoculum from the swab; (d) inoculating the dried surface of a Mueller-Hinton agar (MHA) plate by streaking the swab over the entire sterile agar surface; (e) placing discs containing different range of concentration such as 6.25-100 mg/ml of TiO2nanoparticle and ZnO/GO nano composite separately on top of the inoculated plates and incubated at 30 °C for 24 hours; and (f) evaluating the bacterial activity by measuring the zone of growth inhibition surrounding the well. USE - Method for presenting an antimicrobial activity of Titanium dioxide (TiO2) and Zinc oxide (ZnO)/Graphene oxide (GO) nanomaterial on Enterococcus faecalis MTCC 439. ADVANTAGE - The method is simple, efficient, raw material is cheap and easy to get, and preparation process is green, obtained composite granule particle diameter distribution uniformly and good dispersion. DETAILED DESCRIPTION - Presenting an antimicrobial activity of Titanium dioxide (TiO2) and Zinc oxide (ZnO)/Graphene oxide (GO) nanomaterial on Enterococcus faecalis MTCC 439 involves (a) preparing the inoculum using 24 hours plate cultures of the test bacteria (Enterococcus faecalis MTCC 439); (b) suspending the colonies in 0.85% saline, and comparing the turbidity with the 0.5 McFarland standards to produce a suspension of 1.0 × 108 Colony-forming unit (CFU) mL-1; (c) loading the suspension on a sterile cotton swab that is rotated multiple times and pressed firmly against the inside wall of the tube to remove excess inoculum from the swab; (d) inoculating the dried surface of a Mueller-Hinton agar (MHA) plate by streaking the swab over the entire sterile agar surface; (e) placing discs containing different range of concentration such as 6.25-100 mg/ml of TiO2 nanoparticle and ZnO/GO nano composite separately on top of the inoculated plates and incubated at 30 °C for 24 hours; and (f) evaluating the bacterial activity by measuring the zone of growth inhibition surrounding the well.