▎ 摘　　要

NOVELTY - An immobilized microcystin (MC) degrading enzyme preparing method involves activating graphene oxide with carboxyl activating agent in presence of first solvent to obtain activated graphene oxide, separating activated graphene oxide into solid and liquid, contacting the activated graphene oxide with functionalized iron oxide nanoparticles in presence of second solvent, and separating mixture to obtain solid phase-carrier. MC-degrading enzyme is loaded in the solid-phase carrier. USE - Method for preparing immobilized MC-degrading enzyme that is utilized in degrading algal toxins chosen from MC-LR, MC-YR and MC-RR, where the MC-degrading enzyme is MlrA, MlrB, MlrC or MlrD (all claimed). ADVANTAGE - The method enables preparing immobilized MC-degrading enzyme with high algal toxin degradation rate, degrading algal toxins in cost-effective manner, increasing number and environment tolerance of the enzyme, and controlling reaction process strictly. DETAILED DESCRIPTION - An immobilized microcystin (MC) degrading enzyme preparing method, involves activating graphene oxide with carboxyl activating agent in presence of first solvent to obtain activated graphene oxide, separating activated graphene oxide into solid and liquid, contacting the activated graphene oxide with functionalized iron oxide nanoparticles in presence of second solvent, and separating mixture to obtain solid phase-carrier. MC-degrading enzyme is loaded in the solid-phase carrier. The functionalized iron oxide nanoparticles are amino-functionalized iron oxide nanoparticles, carboxyl-functionalized iron oxide nanoparticles, azide-functionalized iron oxide nanoparticles, or thiol-functionalized iron oxide nanoparticles. The weight ratio of the graphene oxide and functionalized iron oxide nanoparticles is 1-20:1. An INDEPENDENT CLAIM is also included for an immobilized MC-degrading enzyme prepared by the above-mentioned method.