▎ 摘　　要

NOVELTY - Integrated microfluidic device comprises a glass slide, an inlet micro one-way valve, a bottom layer PDMS structure, a bottom layer microfluidic the channel, an outlet micro one-way valve and a top layer PDMS structure. The bottom layer (microfluidic) is set on the bottom layer PDMS structure, the inlet end and the outlet end of the bottom layer PDMS structure are punched. A computer (11) connected with a high-speed camera (12) and a fluorescent microscope (13). The computer is connected with the high speed camera. The fluorescent microscope is fixed on a position of a double microfluidic chip position. The controllable direct current voltage stabilizing source (10) provides the stable direct current working voltage;. USE - Integrated micro-fluidic device for measuring viscosity of cerebrospinal fluid i.e. colorless transparent liquid, present in a ventricle and a subarachnoid cavity of a human body. Uses include but are not limited to Alzheimer's disease, Chiari malformation type 1, hydrocephalus and spinal cord cavern. ADVANTAGE - The integrated micro-fluidic device has high conductivity, high light transmittance, high stability of the flexible graphene film has uniform and stable temperature control to the microfluididic, the temperature is maintained at the temperature near the human body, removing the influence of temperature change to cerebrospinal fluid property, using nano-particle tracking technology to contact detail track information to measure the displacement, so as to reversely calculate the viscosity of the cerebro Spinal fluid, the method is simple and fast, saving sample, and the cost is low. Based on the three points, the device can realize low loss, low cost, non-contact cerebro spinal fluid viscosities measurement. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for the method for measuring viscosity of cerebrospinal fluid by using integrated microfluidic device for measuring cerebral spinal fluid viscosity. DESCRIPTION OF DRAWING(S) - The drawing shows a structure diagram of cerebrospinal fluid viscosity measuring device. 10Controllable direct current voltage stabilizing source 11Computer 12High speed camera 13Fluorescent microscope