▎ 摘　　要

NOVELTY - Graphene-nanofiber hybrid scaffold for tissue engineering comprises nanofibers coated with graphene oxide. The nanofibers have an average diameter of 100 nm to 3 mu m. USE - The scaffold is useful for tissue engineering; for preparing implantable medical device; for directing stem cell differentiation; for treating disease, injury or disorder; and for dedifferentiating lineage committed mammalian cells into iPS cells (all claimed). The disease, injury or disorder includes neurodegenerative disorder, neurological injury, traumatic brain injury, spinal cord injury, peripheral nerve trauma, Alzheimer's disease, Parkinson's disease, Huntington's disease, epilepsy, stroke and dementias. ADVANTAGE - The graphene-nanofiber hybrid scaffold allows for selective stem cell differentiation and better biocompatibility. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are: (1) an implantable medical device comprising the graphene-nanofiber hybrid scaffold; (2) a method of directing stem cell differentiation comprising exposing the scaffold to a culture media comprising stem cells for a period of time sufficient to allow the stem cells to differentiate into cells of interest; (3) a method of treating an injury or disorder comprising implanting the scaffold in a subject, where the scaffold is seeded with stem cells or differentiated stem cells; (4) a method of preparing scaffold comprising contacting nanofibers with a solution of graphene oxide, where the concentration of the graphene oxide is optionally adjusted to control the thickness of the coating; (5) a method of dedifferentiating lineage committed mammalian cells into induced pluripotent stem cells (iPS cells), comprising seeding lineage committed mammalian cells in the scaffold; and exposing the scaffold to a culture medium for a sufficient period of time to allow dedifferentiation of the lineage committed mammalian cells; (6) an iPS cell produced by the method above; and (7) a method of treating a disease or disorder comprising administering iPS cells to a subject.