▎ 摘　　要

NOVELTY - Biosensor comprises base layer and graphene layer. The graphene layer is located on the base layer, the graphene layer is plated with a metal electrode; the metal electrode includes input electrode and output electrode and one input electrode corresponds to one output electrode, and a part of the non-metal electrode on the graphene layer is modified with bridging molecules for connecting biological macromolecules. The bridging molecule includes pyrene or perylene or anthracene anchoring group for connecting with graphene, active ester or disulfide bond or maleic acid glycoside for connecting with biological macromolecule, and at least one linking groups that connect the anchor group to the active ester or disulfide bond or maleic acid glycoside, and the biological macromolecules connected to the bridging molecules are virus antigens or virus antibodies or nucleic acid probes. USE - The biosensor is useful in virus detection system for virus detection (all claimed). ADVANTAGE - The biosensor: realizes the detection of different viruses. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) biosensor comprising a base layer, the base layer is plated with a metal electrode, the metal electrode includes an input electrode and an output electrode, and one input electrode corresponds to one output electrode, each input electrode and its corresponding output electrode are connected by silicon nanowires, the silicon nanowires are modified with bridging molecules for connecting biological macromolecules, the bridging molecule is a nickel ion (Ni2+) probe or maleimide, and the biological macromolecules connected to the bridging molecules are virus antigens or virus antibodies or nucleic acid probes; (2) preparing biosensor comprising either (i) transferring the single-layer graphene generated on the metal surface to the surface of the base material to form the base layer and the graphene layer, (ii) spin-coating photoresist on the graphene layer and etching the shape of the metal electrode by photolithography, (iii) vapor-depositing metal electrode on the position of the metal electrode on the graphene layer to form a device to be modified, where the metal electrode includes: an input electrode and an output electrode, and one input electrode corresponds to one output electrode, (iv) modifying part of the non-metal electrode on the graphene layer of the device to be modified with bridging molecules, and (v) connecting viral antigens or viral antibodies or nucleic acid probes to the bridging molecule, or (i) carrying out surface functionalization of silicon nanowires generated on the substrate, (ii) spin coating photoresist on the substrate and etching the shape of the metal electrode by photolithography technology, (iii) evaporating a metal electrode on the substrate to form a device to be modified, where the metal electrode includes input electrodes and output electrodes, one input electrode corresponds to one output electrode, and each input electrode and its corresponding output electrode are connected by silicon nanowires, (iv) modify bridging molecules on the silicon nanowire of the device to be modified, and (v) connecting viral antigens or viral antibodies or nucleic acid probes to the bridging molecule; (3) virus detection system comprising biosensor, signal generator, signal collector, signal analysis host and display, the signal generator is connected to each input electrode of the biosensor, and sends a trigger electrical signal to each input electrode, the signal collector is connected to each output electrode of the biosensor, collects the output current signal and sends it to the signal analysis host, the output current signal is the current signal obtained before and after the reaction of the virus antigen or virus antibody or the nucleic acid probe used to detect the virus in the biosensor with the sample to be detected, and the signal analysis host analyzes and detects the received output current signal, determines whether there is a virus in the sample to be tested, and sends the analysis and detection result to the display for display; and (4) virus detection using virus detection system comprising sending trigger electric signal to each input electrode of the biosensor signal generator, inputs the sample to be tested into the biosensor, connecting the signal collector to each output electrode of the biosensor, collecting the output current signal and sending to the signal analysis host, the output current signal is the current signal obtained before and after the reaction of the virus antigen or virus antibody or the nucleic acid probe used to detect the virus in the biosensor with the sample to be detected, and the signal analysis host analyzes and detects the received output current signal, determines whether there is a virus in the sample to be tested, and sends the analysis and detection result to the display for display.