▎ 摘　　要

NOVELTY - A hemoglobin detecting method involves preparing a graphene quantum dot solution and hemoglobin solution, mixing and diluting with phosphate buffer to prepare a standard solution. The fluorescence spectrum of the standard solution is measured and the fluorescence intensity is recorded, followed by establishing the working curve. The sample to be tested is mixed with the solution of the graphene quantum dots and the phosphate buffer is added to the test solution to detect the fluorescence spectrum, followed by calculating the amount of hemoglobin in the sample to be tested. USE - Method for detecting hemoglobin based on graphene quantum dot fluorescence. ADVANTAGE - The method enables detecting the hemoglobin with good linear relation, high-sensitivity and wide application prospect. DETAILED DESCRIPTION - A hemoglobin detecting method involves dispersing a graphene quantum dot in a phosphate buffer to obtain a graphene quantum dot solution. Hemoglobin is dissolved in phosphate buffer to obtain hemoglobin solution. The graphene quantum dot solution and hemoglobin solution are thoroughly mixed and diluted with phosphate buffer to prepare a standard solution. The fluorescence spectrum of the standard solution is measured and the fluorescence intensity is recorded, followed by establishing the working curve of hemoglobin concentration based on fluorescence quenching degree ( Delta F) of fluorescence quenching effect of hemicellulose on the graphene quantum dots. The sample to be tested is mixed with the solution of the graphene quantum dots and the phosphate buffer is added to the test solution to detect the fluorescence spectrum, followed by calculating the amount of hemoglobin in the sample to be tested according to the fluorescence quenching degree ( Delta F) and the working curve, where the concentration of graphene quantum dots is 20 mg/L, the concentration of hemoglobin is 0-0.5 mu mol/L.