▎ 摘　　要

NOVELTY - Pumping (M1) fluids through microchannels of a bar-chart microfluidic chip comprises: irradiating a photothermal agent within an inlet reservoir of the bar-chart microfluidic chip with light having a wavelength that is absorbed by the photothermal agent and is converted to heat; and responsive to an increase in vapor pressure within the inlet reservoir due to irradiating the photothermal agent, forcing fluids through the microchannels of the microfluidic chip. USE - The methods are useful for: pumping fluids through microchannels of bar-chart microfluidic chip; and quantitatively immunoassaying an analyte, where the analyte is protein, nucleic acid, metabolite, small molecule, fungus, virus or bacterium (all claimed). ADVANTAGE - The method utilizes the on-chip nanomaterial-mediated photothermal effect as a tunable microfluidic driving force to drive ink bar-charts in a visual quantitative readout fashion and the photothermal bar-chart pumping performance can be adjusted remotely by tuning the irradiation parameters, without the need to change any on-chip parameters and the photothermal bar-chart chip enables point-of-care visual quantitative diagnostics, by forming nanomaterial-mediated photothermal effects-driven bar-chart microchip for visual quantitative immuno-sensing. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) quantitatively immunoassaying (M2) an analyte comprising conjugating a sample with the photothermal agent or the photothermal precursor to form an analyte-conjugate; loading the analyte-conjugate into an inlet reservoir of the photothermal bar-chart microfluidic chip, irradiating the analyte-conjugate to (a) increase vapor pressure within the inlet reservoir of the photothermal bar-chart microfluidic chip and (b) force fluids through many microchannels and quantitatively determining an antibody or antigen concentration in the sample based on a moving distance that the analyte conjugate travels in the plurality of microchannels; and (2) a photothermal bar-chart microfluidic chip comprising a photothermal agent contained within a reservoir of the bar-chart microfluidic chip and a number of micro-channels extending from the reservoir, where irradiating the photothermal agent with light having a wavelength that is absorbed by the photothermal agent causes an increase in vapor pressure within the reservoir and forces fluids through many microchannels, and a distance that the fluids travel in the microchannels is proportional to an amount of photothermal agent. DESCRIPTION OF DRAWING(S) - The figure shows a schematic view of structures of the photothermal bar-chart chip. Inlets of chip (1) Reservoirs of chip (2)