▎ 摘　　要

NOVELTY - Preparing an electrochemical immunosensor based on reduced graphene oxide/prussian blue-gold platinum (rGO/PB-AuPtNPs) nanocomposite materials involves taking 1.5 microliter of rGO/PB-AuPtNPs composite solution and dropping onto the surface of the working electrode, and placing in a 25 degrees C constant temperature incubator for drying. The rGO/PB-AuPtNPs composite solution is formed by mixing reduced graphene oxide/prussian blue (rGO/PB) solution and gold platinum nanoparticles (AuPtNPs) solution in a volume ratio of 1:2. The 5.0 microlieter of hepatitis B surface antibody (HBsAb) is added dropwise to the surface of the modified electrode, and incubated in a refrigerator at 4 degrees C for 12 hours. The bovine serum albumin (BSA) is taken and dropped onto the surface of the modified electrode to eliminate unblocked non-specific active sites, then washed and dried, then stored in a refrigerator at 4 degrees C, an electrochemical immunosensor based on rGO/PB-AuPtNPs nanocomposite materials is prepared. USE - Method for preparing an electrochemical immunosensor based on rGO/PB-AuPtNPs nanocomposite materials for detection of hepatitis B surface antigen (claimed). ADVANTAGE - The method enables to prepared electrochemical immunosensor based on rGO/PB-AuPtNPs nanocomposite materials, that has the advantages of high sensitivity, strong specificity and high accuracy, and can play an important role in the early diagnosis and clinical monitoring of hepatitis B. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for using electrochemical immunosensor based on the rGO/PB-AuPtNPs nanocomposite material for detection of hepatitis B surface antigen, which involves: (A) using the screen-printed electrode as the base electrode, and using an electrochemical workstation to test in 50 microliter, and pH is 7.4 phosphate buffer solution; (B) detecting in the range of potential (-0.5)-0.5 volts by differential pulse voltammetry; (C) using different concentrations of hepatitis B surface antigen to measure, record the peak current changes corresponding to different concentrations of antigen, so as to obtain the corresponding regression equation and correlation coefficient; and (D) using the working curve method to obtain the concentration of hepatitis B surface antigen in the sample to be tested.