• 专利标题:   Preparing Pythium oligandrum graphene material composite biocontrol agent, comprises e.g. adding graphene-based materials into sterilized fungal liquid culture medium, culturing, and washing culture medium with deionized water.
  • 专利号:   CN112877221-A, CN112877221-B
  • 发明人:   ZHENG Q, MAO T, WANG P, FANG Y, JI B, WANG Z, YANG H, ZHU Y
  • 专利权人:   INST BIOLOGY GANSU ACAD SCI
  • 国际专利分类:   A01N059/00, A01N063/30, A01P003/00, C12N001/14, C12R001/645
  • 专利详细信息:   CN112877221-A 01 Jun 2021 C12N-001/14 202154 Pages: 9 Chinese
  • 申请详细信息:   CN112877221-A CN10286825 17 Mar 2021
  • 优先权号:   CN10286825

▎ 摘  要

NOVELTY - Preparing Pythium oligandrum graphene material composite biocontrol agent, comprises (i) adding graphene-based materials to the sterilized fungal liquid culture medium in an amount of 5-100 mg/l, and stirring evenly, (ii) inserting the Pythium oligandrum strain into the medium prepared in step (i), culturing at 28 degrees C, at a speed of 150 revolutions/minute, until a large number of black-gray clusters of flocculent Pythium oligandrum mycelium grow, and the medium is clear and bright, and (iii) taking out the mycelium, washing the culture medium on the mycelium with deionized water. USE - The agent is useful for inhibiting Alternaria solani and Fusarium oxysporum cucumber (claimed). ADVANTAGE - The method is simple, and the production device has low requirement, low cost, suitable for mass production. The composite biocontrol agent has good biocontrol effect, and good popularization. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for Pythium oligandrum graphene material composite biocontrol agent obtained by the method as mentioned above.