▎ 摘　　要

NOVELTY - Microfluidic chip based on dielectric deterministic displacement comprises a micro flow channel (4) having sample inlet, a straight channel and two sample outlets, three groups of electrode array pairs are integrated at the bottom of the straight channel and the three electrode array pairs are focusing electrode group (1), sorting electrode group (2) and separating electrode group (3). The focusing electrode group is used for focusing and signal detection of cells. The sorting electrode group is used for single cell sorting. The separation electrode group is used for single cell separation. The material of the electrode group includes indium tin oxide, carbon, graphene and metal electrode. USE - The chip is useful in device for sorting cell (all claimed), identifying cell type of living cell and phenotype change of cell. ADVANTAGE - The microfluidic chip can sort cell in High-speed and precisely without losing target cell. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) device based on dielectric deterministic displacement comprising a microfluidic chips, a function generator to generate sinusoidal alternating current with different frequencies and voltages, a computer to control the operation of the program and the signal collection and collection of related optical instruments, control the output voltage, frequency, duty cycle and other parameters of the function generator, and control the switch of the relay to control the switch of the circuit, and a fluid drive device comprising an injection pump or a gravity driving device as the source of driving force to drive fluid into the micro channel; and (2) sorting cell based on dielectric deterministic displacement, comprising sampling cell by using a fluid drive device to make the fluid containing cells enter the straight channel through the injection port of the microfluidic chip, (b) connecting the focus electrode group to the output terminal of the function generator through a wire, and periodically apply a high-frequency voltage to the focus electrode group to focus the cells in the fluid to the signal monitoring point of the electrode angle, (c) aligning the optical signal collection point with the monitoring point, detecting optical signal detection, and processing the detected signal by a computer program to determine whether it is a target cell, (d) sorting cell by connecting the trigger relay to the sorting electrode group to output high-frequency voltage, so that the target cell is offset from the center line of the sorting electrode, when it is a target cell, and marking cells, where the program control does not turn on the relay, so that the non-target cells continue to move along the center line of the original focusing electrode group without shifting, when the program determines that it is a non-target cell, and (e) separating cell by applying high-frequency voltage on the separation electrode group to make the fluid in non-target cells and target cells flow to different outlets. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic representation of the microfluidic chip. Focusing electrode group (1) Sorting electrode group (2) Separating electrode group (3) Micro flow channel (4) Signal detection point (5)