▎ 摘　　要

NOVELTY - Analyzing image, comprises (a) preparing a substrate including a cell culture medium on which a biopolymer membrane is formed; (b) preparing a biological sample in which the cell culture medium is included between the graphene and the cell membrane toward the cell membrane of the cultured cells on the biopolymer membrane, and capping the cell so that the graphene covers the cell membrane; (c) performing mass spectrometry imaging or ultra-high vacuum-based imaging on the biological sample to analyze the structure and components of the cell membrane. The step (a) is carried out by depositing silicon nitride thin film on both sides of the substrate; patterning an array of micro-holes on silicon nitride (Si3Ni4) thin film using photo resist on front side of substrate; etching center of back side of substrate to expose array of micro holes; forming biopolymer film on array of micro holes; and injecting cell culture medium onto substrate through array of micro-holes. USE - The method and device are useful for analyzing cell membrane structure and components of living biological sample (all claimed). ADVANTAGE - The method provides in vitro mass spectrometric imaging platform in ultra-high vacuum environment for untreated cells and biological samples for various studies in basic biology, biomedical science, electrochemistry and materials science, and prevents damage of cell membrane and can be performed on components. The device analyzes structure and components of intact cell membranes of living cells using secondary ion imaging analysis technology as well as high-resolution cell structure analysis using electron microscope. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for imaging analysis device comprising a substrate comprising a cell culture medium on which a biopolymer membrane is formed, where the cell culture medium is included between the graphene and the cell membrane toward the cell membrane of the cells cultured on the biopolymer membrane, and the graphene covers the cell membrane a biological sample generating unit generating a biological sample in which the cells are capped; a beam generator configured to scan the biological sample with any one of a primary ion beam and an accelerated electron beam; and an imaging analyzer for collecting secondary ions related to the scanned primary ion beam or secondary electrons related to the scanned accelerated electron beam from the cell membrane to analyze the structure and components of the cell membrane. DESCRIPTION OF DRAWING(S) - The drawing shows a flowchart illustrating a method for analyzing image (Drawing includes non-English language text).