• 专利标题:   Composition for temperature sensors used in various biological applications, comprises graphene oxide, duplex in which DNAzyme strand and peptide nucleic acid (PNA) strand are complementarily linked, and DNAzyme cofactor.
  • 专利号:   EP3792608-A1, JP2021047175-A, US2021080333-A1, KR2021032095-A, KR2239459-B1, EP3792608-B1, JP6970798-B2
  • 发明人:   LEE J, KIM W, YOON S, KIM W G
  • 专利权人:   KOREA RES INST CHEM TECHNOLOGY, KOREA RES INST CHEM TECHNOLOGY, KOREA RES INST CHEM TECHNOLOGY, KOREA RES INST CHEM TECHNOLOGY
  • 国际专利分类:   C12N015/11, C12Q001/6816, G01K003/04, G01K011/12, G01K011/16, C01B032/192, C12Q001/6813, C12Q001/6876, G01N027/414, C09K011/06, G01N031/22
  • 专利详细信息:   EP3792608-A1 17 Mar 2021 G01K-003/04 202128 Pages: 44 English
  • 申请详细信息:   EP3792608-A1 EP196273 15 Sep 2020
  • 优先权号:   KR113372

▎ 摘  要

NOVELTY - Composition for temperature sensors comprises graphene oxide, a duplex in which a DNAzyme strand and a peptide nucleic acid (PNA) strand are complementarily linked, and a DNAzyme cofactor. USE - The composition is useful for temperature sensors (claimed) used as a barcode (on/off) system for sensing a diverse range of temperatures and used in various biological applications. ADVANTAGE - When the DNAzyme/PNA duplex is dissociated at a certain temperature, graphene oxide adsorbs PNA with excellent selectivity and irreversibility, so it is enabled recall of temperature, which can permit delayed color development in the time of need. In addition, the target temperature can be easily and quickly detected with the naked eye through the color change of the colorimetric reagent, and the thermosensor is technically convenient and easy to apply, so it can be used in various biological applications. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) temperature sensing which involves obtaining a mixture containing a duplex by mixing the DNAzyme strand and the PNA strand in which the DNAzyme strand and the PNA strand are complementarily linked; adding graphene oxide to the mixture; and observing the color change of the mixture while heating the mixture; and (2) a kit for temperature sensors comprising a composition including graphene oxide, a duplex in which a DNAzyme strand and a PNA strand are complementarily linked and a DNAzyme cofactor.