▎ 摘　　要

NOVELTY - A cocaine detecting method involves adding a dye-marked oligonucleotide probe into buffer solution, carrying out fluorescent detection of solution, and recording the oligonucleotide probe for fluorescence emission spectrum. Graphene oxide is added as a solid-phase carrier into the solution, to adsorb the oligonucleotide probe onto surface of graphene oxide and form oligonucleotide/graphene oxide compound, and a fluorescence signal of the dye is quenched. Complementary probe is added into the oligonucleotide/graphene oxide compound for reaction followed by adding cocaine for reaction. USE - Method for detecting cocaine. ADVANTAGE - The method enables detecting cocaine with high specificity and sensitivity in a simple and quick manner. DETAILED DESCRIPTION - A cocaine detecting method involves adding a dye-marked oligonucleotide probe into buffer solution, carrying out fluorescent detection of solution, and recording the oligonucleotide probe for fluorescence emission spectrum. Graphene oxide is added as a solid-phase carrier into the solution, to adsorb the oligonucleotide probe onto the surface of the graphene oxide and form an oligonucleotide/graphene oxide compound, and a fluorescence signal of the dye is quenched. Complementary probe is added into the oligonucleotide/graphene oxide compound for reaction, where the double-strand secondary structure is formed through the base combination effect between the complementary probe and the oligonucleotide probe to recover fluorescence, followed by adding cocaine for reaction, where three stem-like secondary structures are formed through the specific effect between the cocaine and the complementary probe, so that the oligonucleotide probe is re-combined to the surface of the graphene oxide and the fluorescence is quenched.