▎ 摘　　要

NOVELTY - A nucleic acid aptamer capable of specifically recognizing alpha-lactalbumin comprising (a) a 78 base pair sequence (SEQ ID NO: 1) fully defined in the specification or base pair sequence of SEQ ID NO: 2, (b) base pair sequence having more than 60% homology with base pair sequence SEQ ID NO: 1 or 2, (c) hybridization sequence of SEQ ID NO: 1 or SEQ ID NO: 2 or (d) RNA sequence transcribed from the base pair sequence of SEQ ID NO: 1 or SEQ ID NO: 2, is new. USE - The nucleic acid aptamer is useful for preparing alpha-lactalbumin detection kit or alpha-lactalbumin molecular probe (claimed) and biosensors for detecting alpha-lactalbumin. ADVANTAGE - The nucleic acid aptamer has small molecular weight, higher affinity and specificity, wide application range or no immunogenicity compared with protein antibodies, can be chemically synthesized, rapidly, qualitatively and quantitatively detects allergen alpha-lactalbumin and is cost-effective and stable. DETAILED DESCRIPTION - A nucleic acid aptamer capable of specifically recognizing alpha-lactalbumin comprising (a) a 78 base pair sequence (SEQ ID NO: 1) fully defined in the specification or base pair sequence of SEQ ID NO: 2, (b) base pair sequence having more than 60% homology with base pair sequence SEQ ID NO: 1 or 2, (c) hybridization sequence of SEQ ID NO: 1 or SEQ ID NO: 2 or (d) RNA sequence transcribed from the base pair sequence of SEQ ID NO: 1 or SEQ ID NO: 2, is new. 5'-agcagcacagaggtcagatggtgctgcgaa 3' (SEQ ID NO: 2). An INDEPENDENT CLAIM is included for method for detecting alpha-lactalbumin using the nucleic acid aptamer, which involves (a) labeling the nucleic acid aptamer with a FAM (RTM: 5-Carboxyfluorescein) fluorophore, (b) mixing the labeled nucleic acid aptamer and graphene oxide in a mass ratio of 1:20, reacting in the dark for 30-35 minutes, adding the sample to be tested, uniformly mixing, reacting for 30-35 minutes and measuring the fluorescence value of the supernatant.