▎ 摘　　要

NOVELTY - Preparing composite film involves weighing 25-50 mg nano-materials containing graphene and carbon nano-tubes are dispersed in 100-200 mL phosphate buffer solution and incubated at room temperature for 30-60 minutes to obtain nano-materials mixed solution. The weight 75-150 mg quenching enzyme is dispersed in the mixed solution of nano-meter material, and the mixture is placed at room temperature for 30-60 minutes. The obtained nano-materials mixed solution is subjected under vacuum freeze drying, and cryopreservation is carried out to obtain desired product. USE - Method for preparing composite film for nano-meter material and induction quenching enzyme (claimed), used in the field of biology, medicine, food and water treatment. ADVANTAGE - The method enables to prepare composite film, which has good effect of anti-bio-film, and is precessed in simple and cos-friendly manner. DETAILED DESCRIPTION - Preparing composite film involves weighing 25-50 mg nano-materials containing graphene and carbon nano-tubes are dispersed in 100-200 mL phosphate buffer solution and incubated at room temperature for 30-60 minutes to obtain nano-materials mixed solution. The weight 75-150 mg quenching enzyme is dispersed in the mixed solution of nano-meter material, and the mixture is placed at room temperature for 30-60 minutes. The obtained nano-materials mixed solution is subjected under vacuum freeze drying, and cryopreservation is carried out to obtain desired product. The composite ultra-filtration membrane casting solution contains 0.1-1 wt.% graphene-based nano-materials and bacterial induction quenching enzyme hybrid particles is mixed with 80-85wt.% organic solvent, and subjected under ultrasound for 15-30 minutes. The composite solution is mixed with 0.1-1 wt.% polymer additive, and subjected under stirring condition for 30 minutes to obtain polymer macromolecule.