• 专利标题:   New fluorescent aptamer sensor useful for detecting okadaic acid, obtained by performing self-assembly of nucleic acid aptamer that recognizes okadaic acid with end labeled with fluorescent group and graphene oxide.
  • 专利号:   CN111537483-A
  • 发明人:   WU W, YANG Q, WANG Q
  • 专利权人:   UNIV QINGDAO AGRIC
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN111537483-A 14 Aug 2020 G01N-021/64 202074 Pages: 8 Chinese
  • 申请详细信息:   CN111537483-A CN10380992 08 May 2020
  • 优先权号:   CN10380992

▎ 摘  要

NOVELTY - A fluorescent aptamer sensor for detecting okadaic acid, obtained by performing self-assembly of a nucleic acid aptamer that recognizes okadaic acid with 5' end labeled with a fluorescent group and graphene oxide, is new. The nucleic acid aptamer is okadaic acid aptamer. The sequence of the aptamer comprises 40 base pair sequence (SEQ ID NO: Not defined) fully defined in the specification. The graphene oxide is used as a fluorescence quencher. USE - The fluorescent aptamer sensor is useful for detecting okadaic acid (claimed). ADVANTAGE - The sensor detects okadaic acid simply and cost-effectively with high specificity of 0.5-200 ng/ml. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for preparing the fluorescent aptamer sensor for detecting okadaic acid, which involves heating okadaic acid aptamer with a concentration of 1 mu M at 95 degrees C for 5 minutes, cooling to room temperature, adding 20 mu l okadaic acid aptamer to 20 mu l graphene oxide with concentration of 5-25 mu g/ml, shaking at room temperature, incubating for 10 minutes to obtain okadaic acid aptamer and graphene oxide to obtain okadaic acid aptamer-graphene oxide complex, which is the fluorescent aptamer sensor for detecting okadaic acid.