▎ 摘　　要

NOVELTY - The method involves taking germ culture liquid, and centrifuging for 4000 revolution per minute at 5 minutes. An in-situ formation Raman enhance substrate is modified with glucose oxidase, anti-antibody of Escherichia coli and graphene oxide. The silver nano seed, glucose oxidase catalytic, glucose, hydrogen peroxide and silver ion are mixed and reacted to promote silver nano seed growing. The silver nanoparticle is formed, and a Raman detection report module is used to enhance Raman signal intensity and Escherichia coli concentration to detect Escherichia coli in water. USE - In-situ formation Raman enhance substrate water Escherichia coli detection method. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view illustrating an in-situ formation Raman enhance substrate water Escherichia coli detection method. '(Drawing includes non-English language text)'