▎ 摘　　要

NOVELTY - Three-dimensional graphene/silver nanoparticle composite nano-tag material is obtained by depositing three-dimensional graphene and silver nanoparticles jointly on a substrate. USE - Three-dimensional graphene/silver nanoparticle composite nano-tag material is used for performing electrochemical sensing detection method of lncRNA molecules (claimed). ADVANTAGE - The method has advantages in the aspects of specific molecule identification, selectivity, detection limit, precision, biocompatibility and low toxicity, and has good application potential in lung cancer detection. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing a nano tag material, which involves: (a) preparing three-dimensional graphene, and transferring the three-dimensional graphene onto a substrate to obtain a three-dimensional graphene modified substrate; (b) preparing silver nano particles by a chemical reduction method, and preparing the silver nano particles into an electrolyte solution; and (c) taking the substrate modified by the three-dimensional graphene as an electrode to carry out electrodeposition in the electrolyte solution for obtaining the three-dimensional graphene/silver nanoparticle composite nano-marking material; and (2) a method for performing electrochemical sensing detection method of long non-coding RNA (LncRNA) molecules, which involves: (a) modifying the thiolated capture probe on the surface of the three-dimensional graphene/silver nanoparticle composite nano-label material to obtain a three-dimensional graphene/silver nanoparticle/capture probe nano-label material; (b) mixing the three-dimensional graphene/silver nanoparticle/capture probe nano-label material with solutions of four hairpin probes and a solution to be detected, where solution to be detected is preferably a serum sample, and performing shake incubation, where capture probe comprises a sequence 0, and aiming at LncRNA molecules with the sequence of 1, 2, 3, the sequences of the four hairpin probes are hairpin probe 1 of 0', 1', 2', 3', 4, 3 and 2, hairpin probe 2 of 4, 2, 3, 4', 3' and 5, hairpin probe 3 of 5', 6 and 5, and hairpin probe 4 of 5, 6' and 5'; (c) separating the treated solution centrifugally to obtain nano-labeled probe molecules; and (d) loading the nano-labeled probe molecules on an indium tin oxide (ITO) electrode, performing electrochemical test by taking ITO as a working electrode, and detecting LncRNA according to electrochemical response.