• 专利标题:   Confirming introduction of foreign genes into cells by processing graphene oxide sensor in which water-soluble polymer and fluorescent conjugated probe are bound to cell into which foreign gene is introduced and checking emission.
  • 专利号:   KR2021031406-A, US2021102241-A1, KR2533992-B1
  • 发明人:   MIN D, LIM Y J, YIM Y
  • 专利权人:   UNIV SEOUL NAT R DB FOUND, UNIV SEOUL NAT R DB FOUND
  • 国际专利分类:   C01B032/23, C12N005/071, C12N005/09, C12Q001/6841, C12Q001/6876, C12N015/113, C12N009/22, C12Q001/6823
  • 专利详细信息:   KR2021031406-A 19 Mar 2021 C12Q-001/6841 202130 Pages: 42
  • 申请详细信息:   KR2021031406-A KR116011 10 Sep 2020
  • 优先权号:   US898039P, KR116011

▎ 摘  要

NOVELTY - Method for confirming introduction of foreign genes into cells involves introducing a foreign gene into a target cell, processing a graphene oxide sensor in which a water-soluble polymer and a fluorescent conjugated probe are bound to the cell into which the foreign gene is introduced, and checking fluorescence emission in the cells, where the probe specifically binds to a substance produced in the target cell by the introduction of the foreign gene. USE - The method is useful for confirming introduction of foreign genes into cells. ADVANTAGE - The method simply and accurately confirms introduction of foreign genes and does not require an additional sampling process. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for producing a foreign gene transduced cell, which involves introducing a foreign gene into a target cell, treating a graphene oxide sensor in which a water-soluble polymer is bonded to a carboxy group portion on the surface to a cell into which the foreign gene is introduced, and bounding fluorescent conjugated probe to the remaining portion of surface to which the water-soluble polymer is not bonded, confirming fluorescence emission in the cells, and culturing a cell whose light emission has been confirmed, where the probe specifically binds to a substance produced in the target cell by the introduction of the foreign gene.