▎ 摘　　要

NOVELTY - A superoxide dismutase modified vanadium nitride paste electrode sensor preparing method involves adding nano vanadium nitride, graphene oxide, N-ethyl-3-methylpyridine hexafluorophosphate, liquid paraffin and petroleum ether in the agate mortar and grinding to obtain nano-vanadium nitride/graphene/N-ethyl-3-methylpyridine hexafluorophosphate mixture carbon paste. The resulting carbon paste is placed in a glass tube, compacted, dried, polished and washed to obtain a nano vanadium nitride/graphene composite paste electrode. The reactor is added with anhydrous ethanol and gamma -aminopropyltriethoxysilane, mixed, taken out the electrode, washed, dried, placed in a glutaraldehyde solution and dried to obtain an aminopropyltriethoxysilane modified electrode. The reactor is added with phosphate buffer, bovine serum albumin and superoxide dismutase, stirred and dissolved, which is applied to the aminopropyltriethoxysilane modified electrode and dried to obtain the finished product. USE - Method for preparing superoxide dismutase modified vanadium nitride paste electrode sensor. ADVANTAGE - The method enables preparing the superoxide dismutase modified vanadium nitride paste electrode sensor with wide electrochemical window, high carbon paste electrode conductivity, high sensitivity and better selectivity in simple and cost-effective manner. DETAILED DESCRIPTION - A superoxide dismutase modified vanadium nitride paste electrode sensor preparing method involves adding 42-45 mass% nano vanadium nitride, 20-25 mass% graphene oxide, 12-18 mass% N-ethyl-3-methylpyridine hexafluorophosphate, 8-12 mass% liquid paraffin and 6-10% petroleum ether in the agate mortar with sum of the mass percentages of each component of 100% and uniformly grinding to obtain nano-vanadium nitride/graphene/N-ethyl-3-methylpyridine hexafluorophosphate mixture carbon paste. The resulting carbon paste is placed in a glass tube with a wire of 3mm, compacted, dried, polished with polishing powder, polished and washed with deionized water to obtain a nano vanadium nitride/graphene composite paste electrode. The reactor is added with 74-78 mass% anhydrous ethanol and 22-26 mass% gamma -aminopropyltriethoxysilane, uniformly mixed, placed the nano-vanadium nitride/graphene composite paste electrode at room temperature for 4-6 hours, raised the temperature to 50/|2 degrees C for 6 hours, taken out the electrode, washed with absolute ethanol, dried, and placed in a glutaraldehyde solution with a mass concentration of 15%, put in ultrasound for 20-30 minutes, taken out and dried to obtain an aminopropyltriethoxysilane modified electrode. The reactor is added with 98-99 mass% phosphate buffer solution having a pH of 7.0, 0.4-1.5 mass% bovine serum albumin and 0.4-1.5 mass% superoxide dismutase with sum of the mass percentages of each component of 100%, stirred and dissolved to obtain superoxide dismutase fixing solution, which is applied to the aminopropyltriethoxysilane modified electrode with amount of the dripping of 15-20 mu L and dried to obtain the finished product.