▎ 摘　　要

NOVELTY - Apparatus comprises ion filter coupled to select a sample ion from an ionized sample supply, energy reduction cell coupled to receive the selected sample ion and reduce a kinetic energy of the sample ion, substrate positioned to receive the sample, ion transport module coupled to receive the sample ion from the energy reduction cell and transport the sample ion to the substrate, imaging unit arranged to imagethe sample located on the substrate, where the substrate is arranged in an analysis location, the imaging includes an emitter coupled to direct a charged particle beam toward the sample and a detector arranged to detect charged particles emitted from or transmitting through the sample. USE - Apparatus used for preparing and delivering biological samples for charged particle analysis of proteins, DNA and macromolecular assemblies, for structural and charge-based analysis. ADVANTAGE - The apparatus provides the flexibility in sample type and size as desired, and enables the preparation and delivery of biological samples for charged particle analysis in a simple and cost-effective manner. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: 1. Method for preparation and delivery of biological samples for charged particle analysis, involving: a. ionizing a sample supply, filtering, with a quadrupole mass filter, a target sample ion from the ionized sample supply; b. depositing the target sample ion onto a substrate and imaging, with charged particles, a target sample on the substrate, the substrate located in an analysis location, where the target sample is a neutralized target sample ion; and 2. Direct electron imaging system, comprises a sample preparation and validation subsystem coupled to receive a sample supply and provide a target sample ion, transportation and deposition subsystem coupled to receive the target sample ion and deposit the target sample ion onto a substrate, imaging subsystem coupled to image the target sample ion and a controller coupled to control the sample preparation and validation, transportation and deposition and imaging subsystems, the controller coupled to memory including code that, when executed, causes the system to ionize the sample supply, filter, with a quadrupole mass filter, the target sample ion from the ionized sample supply, deposit the target sample ion onto the substrate and image, with electrons, a target sample on the substrate, the substrate located in an analysis location, wherein the target sample is a neutralized target sample ion.