▎ 摘　　要

NOVELTY - Preparing immunosensor based on titanium dioxide doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle involves polishing glassy carbon electrode with a diameter of 3.0-5.0 mm into a mirror surface with 1.0 mu m, 0.3 mu m and 0.05 mu m alumina polishing powder, and ultrasonically cleaning in absolute ethanol. 6.0 mu L of 0.5-4.0mg/mL of titania-doped graphene-loaded sea cucumber-like gold-palladium core-shell nanoparticles dispersed droplets are taken and applied to the surface of the electrode, dried at room temperature, and electrode surface is rinsed with ultrapure water, and dried. 6.0 mu L of 5.0-15.0 mu g / mL of human epididymal protein 4 antibody is added to the surface of the electrode, and then placed in a refrigerator at 4 degrees C to dry. USE - Method for preparing immunosensor based on titanium dioxide doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle for detecting human epididymis protein 4 antigen, where ovarian cancer marker is human epididymal protein 4 antigen HE4, sugar chain antigen CA125 (all claimed). ADVANTAGE - The method enables to prepare immunosensor based on titanium dioxide doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle, which has high specificity, high sensitivity and low detection limit and detection of ovarian diseases has important scientific significance and application value. DETAILED DESCRIPTION - 3.0 mu L of 1.0-2.0 wt.% bovine serum albumin solution is added to the electrode surface to block the non-specific active site on the surface of the electrode, surface of the electrode is rinsed with pH 7.0 phosphate buffer, and placed in a refrigerator at 4 degrees C to dry. A series of different concentrations of human epididymis protein 4 antigen solution of 6.0 mu L, 50 fM-80nM is applied to the surface of the electrode, and placed in a refrigerator at 4 degrees C for 30-40 min. The working electrode of a current-type immunosensor is prepared by drying in a refrigerator at 4 degrees C. An INDEPENDENT CLAIM is included for use of immunosensor based on titanium dioxide doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle is claimed for detecting human epididymal protein 4 antigen, where electrochemical workstation is used to test with a three-electrode system, the saturated calomel electrode is a reference electrode, and the platinum wire electrode is an auxiliary electrode, prepared sensor is a working electrode and is tested in a phosphate buffer solution of 10 mL, 50 mmol/L, pH 5.84-8.04, analyte is detected by the time-current method, the input voltage is -0.4 V, the sampling interval is 0.1 s, and the running time is 400 s, when the background current tends to be stable, 10 mu L of a 5 mol/L aqueous solution of hydrogen peroxide is injected into a 10 mL, 50 mmol/L, pH 7.4 phosphate buffer solution every 50 s to record the current change.