▎ 摘　　要

NOVELTY - Preparing hepatitis B marker immune sensor based on graphene quantum dots with copper oxide with reduced graphene oxide comprises (i) polishing alumina powder on glassy carbon electrode and cleaning; (ii) immersing pretreated electrode in chloroauric acid solution and scanning; (iii) immersing treated electrode in thioglycolic acid and drying; (iv) adding graphene quantum dots with copper oxide with reduced graphene oxide solution to electrode surface; (v) adding hepatitis B antibody into electrode surface; and (vi) adding bovine serum albumin into electrode surface and cooling. USE - The hepatitis B marker immune sensor based on graphene quantum dots with copper oxide with reduced graphene oxide is useful for detecting hepatitis B markers (claimed). ADVANTAGE - The sensor has strong specificity, high sensitivity and low detection limit. DETAILED DESCRIPTION - Preparing hepatitis B marker immune sensor based on graphene quantum dots with copper oxide with reduced graphene oxide comprises (i) polishing alumina powder on diameter of 3-5 mm glassy carbon electrode and cleaning with ultra-pure water; (ii) immersing pretreated electrode in 10 ml (1 mmol/liter) chloroauric acid solution, scanning at constant voltage of -0.2 V for 30 seconds to obtain surface of electrodeposited gold modified electrode, rinsing electrode with ultrapure water and drying; (iii) immersing treated electrode in 10 ml (10 mmol/liter) thioglycolic acid, reacting at room temperature for 24 hours, rinsing surface of the electrode with ultrapure water and drying at room temperature; (iv) adding 6 mu l (2-4 mg/ml) graphene quantum dots with copper oxide with reduced graphene oxide solution to the electrode surface, drying at room temperature, rinsing electrode surface with ultrapure water and drying at room temperature; (v) adding 6 mu l (8-12 mu g/ml) hepatitis B antibody into electrode surface, rinsing with ultrapure water rinse and cooling at 4 degrees C; and (vi) adding 3 mu l (1-2 mg/ml) bovine serum albumin into electrode surface to block non-specific active sites on surface of electrode, rinsing electrode surface with ultrapure water, drying in air, cooling at 4 degrees C in refrigerator A series of different concentrations of antigen solution of adding 6 mu l (5-120 ng/ml) hepatitis B drop wise, rinsing surface of electrode with ultrapure water, drying and cooling in refrigerator at 4 degrees C. An INDEPENDENT CLAIM is also included for detecting hepatitis B markers by graphene quantum dots with copper oxide with reduced graphene oxide, comprising (A) taking three-electrode system to carry out the electrochemical workstation, using saturated calomel electrode as reference electrode, platinum wire electrode as auxiliary electrode, sensor as working electrode, preparing working electrode in 10 ml at 50 mmol/liter and using salt buffer solution at pH 5.1-8 for testing; (B) testing with time-current method, where input voltage is -0.4V, sampling interval is 0.1seconds and running time is 400seconds; and (C) injecting10 mu l , (5 mol/liter) hydrogen peroxide solution into 10 ml (50 mmol/liter) with pH 7.4 phosphate buffer solution for every 50 seconds when background current tends to stabilize and recording change of current.