▎ 摘　　要

NOVELTY - Preparation of graphene oxide-chitosan/nano-gold electrochemical immunosensor involves preparing solid particle dispersion using graphene oxide and chitosan solution, mixing solid particle dispersion and chitosan solution, dispersing to form drip coating liquid, adding drip coating liquid on gold electrode, drying, placing gold electrode in colloidal gold solution to obtain graphene oxide-chitosan/nano-gold modified electrode, dripping antibody Ab1 solution onto surface of modified electrode, heat preserving, applying diluted bovine serum albumin solution on modified electrode, heat-preserving, applying myocardial marker antigen silver on modified electrode, heat-preserving to obtain graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin/silver modified electrode, applying 5-8 mu L enzyme-labeled antibody Ab2 solution to surface of graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin/silver modified electrode and heat-preserving for 0.5-1 hour. USE - Preparation of immunosensor for testing detection performance of myocardial markers (all claimed). ADVANTAGE - The prepared immunosensor is simple, and effective and can quickly and accurately detect myocardial markers of different concentrations. DETAILED DESCRIPTION - Preparation of graphene oxide-chitosan/nano-gold electrochemical immunosensor involves preparing solid particle dispersion using 1.0-5.0 mg/mL graphene oxide and 0.25-2 %mass chitosan solution, mixing solid particle dispersion and the chitosan solution, ultrasonically dispersing to form drip coating liquid where concentration of the solid particle dispersion of graphene oxide in the drip coating liquid is 0.9-4.5 mg/mL, and the chitosan solution is 0.23-1.8 %mass, ultrasonically dispersing drip coating liquid and adding 5-8 mu L drip coating liquid on the gold electrode and drying overnight, placing gold electrode coated with the drop coating solution having thickness of 20-40 nm in colloidal gold solution to obtain a graphene oxide-chitosan/nano-gold modified electrode, and drying overnight, preparing phosphate buffer solution having pH of 7.0-9.0, diluting coated antibody Ab1 of myocardial marker with phosphate buffer solution to obtain antibody Ab1 solution where concentration of coated antibody Ab1 after dilution is 0.2-1.0 mg/ml, dripping 5-8 mu L antibody Ab1 solution onto the surface of modified electrode, heat preserving at 4 degrees C for 6-12 hours to obtain graphene oxide-chitosan/nano-gold/antibody Ab1 modified electrode, adding 0.5-5.0 %mass phosphate buffer solution to bovine serum albumin solution to obtain diluted bovine serum albumin solution, applying 5-8 mu L diluted bovine serum albumin solution on graphene oxide-chitosan/nano-gold/antibody Ab1 modified electrode, heat-preserving at 4 degrees C for 6-12 hours, drying to obtain graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin modified electrode, applying 5-8 mu L myocardial marker antigen silver on the surface of graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin modified electrode, heat-preserving at 37 degrees C for 0.5-1 hour to obtain graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin/silver modified electrode, adding phosphate buffer solution to enzyme-labeled antibody Ab2 of myocardial marker to obtain enzyme-labeled antibody Ab2 solution having concentration of 0.1-0.3 mg/ml, applying 5-8 mu L enzyme-labeled antibody Ab2 solution to surface of graphene oxide-chitosan/nano-gold/antibody Ab1/bovine serum albumin/silver modified electrode and heat-preserving at 37 degrees C for 0.5-1 hour. INDEPENDENT CLAIMS are included for the following: (1) graphene oxide-chitosan/nano-gold electrochemical immunosensor; and (2) method of testing detection performance of cardiac markers, which involves performing electrolysis to detect hydrogen peroxide by cyclic voltammetry using the graphene oxide-chitosan/nano-gold electrochemical immunosensor as working electrode, saturated silver/silver chloride electrode as reference electrode, platinum wire as counter electrode and phosphate buffer solution having pH of 8.