▎ 摘　　要

NOVELTY - An enzymatic glucose sensor preparation method involves preparing functional graphene by adding ionic liquid with graphite oxide water solution, adding potassium hydroxide to obtain functionalized graphite, preparing platinum nano-particles, combining obtained modified graphene with platinum nano-particles, decorating the graphene onto a glassy carbon electrode, combining with glucose oxidase and horseradish peroxidase, and drying the substances to enable the electrode surface to form a layer of uniform composite membrane to obtain a finished product. USE - Method for preparing an enzymatic glucose sensor. ADVANTAGE - The method enables preparing an enzymatic glucose sensor with high biocompatibility, sensitivity and stability in an inexpensive, simple and rapid manner. DETAILED DESCRIPTION - An enzymatic glucose sensor preparation method involves preparing functional graphene by adding ionic liquid with graphite oxide water solution, treating ultrasonically for 50-70 minutes to obtain uniformly dispersed mixture, adding 10 mg potassium hydroxide, treating ultrasonically for 20-40 minutes to obtain transparent and uniform solution, refluxing and reacting resultant product for 24 to 48 hours to obtain functionalized graphite. The obtained product is centrifuged, followed by washing by ethanol and water, drying under vacuum, and preparing platinum nano-particles with solution hydrogen-platinum-chloride, heating to boiling point temperature, adding sodium citrate water solution, and keeping the boiling point temperature for 3 hours to obtain platinum nano-particles. The obtained modified graphene is combined with platinum nano-particles to promote electron transfer, followed by decorating the graphene onto a glassy carbon electrode by implementing layer and layer self-assembly method, utilizing biological signal sensitization amplification effect, combining with glucose oxidase and horseradish peroxidase, and drying the substances at 4 degrees C for 24 hours to enable the electrode surface to form a layer of uniform composite membrane to obtain a finished product.