▎ 摘　　要

NOVELTY - Composition comprises graphene oxide, duplex molecular beacon and DNAzyme cofactor, where the duplex molecular beacon is formed by complementarily conjugating the first single strand containing the target nucleic acid binding sequence and the second single strand containing the DNAzyme sequence at least in part, and the second single strand is separated from the first single strand and adsorbed to graphene oxide to form a graphene oxide complex in the presence of the target nucleic acid. USE - The composition is useful for detecting nucleic acid with an amplified detection signal. ADVANTAGE - The composition is capable of easy and fast point-of-care testing (POCT) and detecting a target nucleic acid with high efficiency by amplifying a detection signal. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) detecting a target nucleic acid with an amplified detection signal, where the duplex molecular beacon is formed by complementarily conjugating the first single strand containing the target nucleic acid binding sequence and the second single strand containing the DNAzyme sequence at least in part, comprising (i) obtaining a mixture by mixing a sample containing a target nucleic acid and a duplex molecular beacon, (ii) adding graphene oxide to the mixture, and (iii) obtaining a concentrated graphene oxide complex by filtering or separating the mixture; and (2) a kit containing the composition.