▎ 摘　　要

NOVELTY - A single stranded target polynucleotide sequence and single stranded capture polynucleotide sequence non-hybridization detecting device comprises a glassy carbon electrode coated with chitosan-graphene nanosheet material, where the chitosan-graphene nanosheet material is prepared using an approximately 0.005(w/v)% graphene and 0.005 (w/v)% chitosan solution, and the capture polynucleotide is attached to the chitosan-graphene nanosheet material. USE - Device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence (claimed) and for use in bioinformatics applications such as functional genomics and homology searching. ADVANTAGE - The device has better specificity and sensitivity, and can be manufactured in an inexpensive and rapid manner. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following: (1) a method for manufacturing a single stranded target polynucleotide sequence and single stranded capture polynucleotide sequence non-hybridization detecting device (2) a method for detecting non-hybridization of single stranded target polynucleotide sequence and single stranded capture polynucleotide sequence.