• 专利标题:   Preparation of cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor for detecting T-2 toxin, involves adding cubic palladium-cuprous oxide-graphene oxide to polished electrode, adding toxin antibody and processing.
  • 专利号:   CN105181775-A, CN105181775-B
  • 发明人:   CAO W, DU B, FAN D, HU L, MA H, PANG X, SUN X, WEI Q
  • 专利权人:   UNIV JINAN
  • 国际专利分类:   G01N001/32, G01N001/34, G01N001/38, G01N027/32, G01N033/569
  • 专利详细信息:   CN105181775-A 23 Dec 2015 G01N-027/32 201638 Pages: 7 English
  • 申请详细信息:   CN105181775-A CN10660598 14 Oct 2015
  • 优先权号:   CN10660598

▎ 摘  要

NOVELTY - Alumina is added to glassy carbon electrode, polished, ultrasonically cleaned and dried. Cubic palladium-octahedral cuprous oxide-graphene oxide solution is added to the electrode surface, dried and toxin antibody is dripped on surface of electrode. Resultant material is heat-preserved, cleaned, and bovine serum albumin is contacted with the resultant material, heat-preserved and cleaned. 6 mu L 0.0001-10 mu g/mL T-2 toxin antigen is then added, processed and stored in refrigerator at 4 degrees C to obtain cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor. USE - Preparation of cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor for detecting T-2 toxin (claimed) in foodstuff. ADVANTAGE - The method efficiently provides cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor having excellent electrochemical catalytic electron transfer capability and sensitivity. DETAILED DESCRIPTION - Alumina having size of 1 mu m, 0.3 mu m or 0.05 mu m is added to glassy carbon electrode, polished, and ultrasonically cleaned using ultrapure water and ethanol, and dried by nitrogen. 6 mu l 1-2 mg/ml Cubic palladium-octahedral cuprous oxide-graphene oxide solution is added to the electrode surface, dried and 6 mu l 5-20 mu g/mL T-2 toxin antibody is dripped on the surface of the electrode. The resultant material is heat-preserved at 4 degrees C for 1 hours, cleaned, and 3 mu l 5-20 mg/mL solution of bovine serum albumin is contacted with the resultant material, heat-preserved at 4 degrees C for 1 hours, and cleaned. 6 mu L 0.0001-10 mu g/mL T-2 toxin antigen is then added, processed and stored in refrigerator at 4 degrees C to obtain cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor. An INDEPENDENT CLAIM is included for method of detecting T-2 toxin using cubic palladium-octahedral cuprous oxide-graphene oxide T-2 toxin sensor.