▎ 摘　　要

NOVELTY - Nano drug carrier comprises albumin molecules as a shell coating on the graphene forming nano composite particles, where the albumin-graphene, albumin-albumin and graphene-graphene in nano composite particles are connected by disulfide bonds. USE - The nano drug carrier is useful for loading tumor chemotherapy drugs or photosensitizer drugs by forming drug-loaded nano particles and tumor targeted therapy material (claimed). ADVANTAGE - The nano drug carrier has reduced stimulus response (claimed), high drug loading capacity; and can lower the irritation of reducing agents. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparing nano drug carrier comprising (I) taking oil-in-water microemulsion and water-in-oil type microemulsion as nano reactor, albumin and graphene as raw materials and cystamine as a linker (Ia) preparing microemulsion of carboxy group-containing graphene by taking aqueous solution of a graphene having carboxy groups on its surface, where the graphene concentration is 0.05-20 mg/ml, uniformly mixing graphene aqueous solution with cyclohexane, Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol, (Ib) preparing albumin microemulsion by preparing aqueous albumin, where the albumin concentration is 0.05-30 mg/ml and uniformly mixing albumin aqueous solution with cyclohexane, Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol, (Ic) preparing cystamine microemulsion by preparing aqueous cystamine, where the cystamine concentration is 0.05-5 mg/ml and uniformly mixing cystamine aqueous solution with cyclohexane, Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol, and (Id) preparing 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) or EDC/N-hydroxysuccinimide (NHS) by preparing 0.02-10 mg/ml aqueous EDC solution and aqueous EDC/NHS solution, where the mass ratio of EDC and NHS is 1:5-1:1, uniformly mixing aqueous EDC or EDC/NHS solution with cyclohexane, Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol, where the volume ratio of Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol is 1:1 and 2:1, the volume ratio of the mixed solution of Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol to cyclohexane is 2:3 and 1:3, the volume ratio of the cyclohexane, Triton X-100 (RTM: Octyl phenol ethoxylate) and N-hexanol with aqueous raw materials and ensuring that there is no turbidity; and (II) (IIa) uniformly mixing aqueous EDC or EDC/NHS microemulsion with graphene microemulsion, oscillating at room temperature for 15-60 minutes for activating surface graphene carboxy groups, then adding cystamine microemulsion, shaking at room temperature for 0.5-5 hours for bonding graphene between each other to disulfide bonds, where the mixed microemulsion is labeled as microemulsion A and the volume ratio of EDC microemulsion or EDC/NHS microemulsion, graphene microemulsion and cystamine microemulsion is 2:1:0.5-1:3:0.5, (IIb) adding EDC microemulsion or EDC/NHS microemulsion into bovine serum albumin (BSA) microemulsion, where the volume ratio of BSA microemulsion and EDC microemulsion or EDC/NHS microemulsion is 5:0.1-1:2, oscillating at room temperature for 15-60 minutes, where the mixed microemulsion is labeled as microemulsion B, adding EDC microemulsion or EDC/NHS microemulsion to microemulsion A, where the volume ratio of microemulsion A and EDC/NHS microemulsion is 5:0.1-5:2, oscillating at room temperature for 15-60 minutes, to obtain micro emulsion C, uniformly mixing microemulsion B, microemulsion C and cystamine Microemulsion in the volume ratio of 6:6:1-6:1:1, oscillating at room temperature for 0.5-5 hours, for formation of disulfide bond between albumin-graphene, and albumin-albumin, to obtain micro emulsion D, (IIc) adding methanol, ethanol, isopropanol, butanol, acetone and/or tetrahydrofuran dropwise to microemulsion D, carrying out ultrasonic oscillation treatment for 0.5-2 hours, where the volume ratio of solvent and microemulsion D is 0.025:1-0.2: 1, (IId) centrifuging step (IIc) product, dispersing the precipitate in absolute ethanol, carrying out ultrasonic treatment for 1-5 minutes, centrifuging (3-5 times), washing (3-5 times) resulting precipitate with deionized water, to obtain albumin-coated graphene nano composite particles.