▎ 摘　　要

NOVELTY - Nanoenzyme comprises nano-carbon used as carrier, and palladium-iron double-metal cluster dispersed by atom level is the nano-carbon loaded palladium-iron double-metal cluster nano-enzyme formed by active centre. USE - Nanoenzyme for applying as antibacterial agent in antibacterial product solution for inhibiting bacteria such as colon Bacillus (all claimed). ADVANTAGE - The nanoenzyme has significantly improved enzyme catalytic activity, simple and easy to operate preparation process, easy to control reaction process, without external light, electricity, under the acidic condition, the room temperature can catalyze the oxygen decomposition in air to generate hydroxyl free radical and hydroxy, to effectively kill bacteria, exhibits excellent catalytic antibacterial performance, and has wide application prospect in the future antibacterial field. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: a method for preparing the nanoenzyme, which involves dispersing the nano-diamond-graphene composite material uniformly in water to obtain suspension, adjusting pH of system to 10, under the stirring, the pH as 4 of palladium salt and iron salt mixed solution at uniform speed drop into the suspension, keeping stirring for 1 hour at 100?oC, filtering, washing, drying to obtain the precursor, reducing the obtained precursor at 500?oC under hydrogen condition for 2 hours to obtain the product; and#a method for applying the nanoenzyme, which involves, in the bacteria suspension, adding nano-carbon loaded palladium iron double metal cluster nano-enzyme.