• 专利标题:   Preparing three-dimensional annular cell scaffold for culturing cells, by using chemical vapor deposition to grow graphene on copper/nickel template, or blending conductive material and fiber, or printing conductive hydrogel and/or aerogel.
  • 专利号:   CN111944750-A
  • 发明人:   CHENG G, HAN F, MA X, HAO Y
  • 专利权人:   CHINESE ACAD SCI SUZHOU NANOTECH NANO
  • 国际专利分类:   C12N013/00, C12N005/0775, C23C016/01, C23C016/26, D01F001/09
  • 专利详细信息:   CN111944750-A 17 Nov 2020 C12N-005/0775 202001 Pages: 18 Chinese
  • 申请详细信息:   CN111944750-A CN10841667 20 Aug 2020
  • 优先权号:   CN10841667

▎ 摘  要

NOVELTY - Method for preparing three-dimensional annular cell scaffold, involves using a chemical vapor deposition method to grow graphene on a copper/nickel template to prepare a three-dimensional annular cell scaffold, or blending conductive material and fiber to form conductive fibers, and using the template method for processing to obtain the three-dimensional annular cell scaffold, or printing the conductive hydrogel and/or conductive aerogel by a three-dimensional printing method to prepare the three-dimensional ring cell scaffold. USE - The method is useful for preparing three-dimensional annular cell scaffold used for culturing cells, where the cells are mesenchymal stem cells. ADVANTAGE - The three-dimensional annular cell scaffold has precise and controllable size and excellent conductivity; At the same time, the present invention uses radio stimulation to inoculate the three-dimensional circular cell scaffold with mesenchymal stem cells, which is simple and convenient, solves the trouble of wire connection, reduces the problems of mesenchymal stem cell flow in the tissue and low survival rate and cell transplant damage, and achieves controllable cell behavior. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a three-dimensional circular cell scaffold prepared by the above method; and (2) a method for culturing mesenchymal stem cells, which involves providing the three-dimensional circular cell scaffold, inoculating the mesenchymal stem cells on the three-dimensional circular cell scaffold, performing radio-stimulation on the three-dimensional circular cell scaffold inoculated with the mesenchymal stem cells with a primary coil with electrical signals, and regulating the proliferation and differentiation of the mesenchymal stem cells.