▎ 摘　　要

NOVELTY - Sensitivity-controllable lead ion-induced double-amplified electrochemiluminescence detection involves preparing complementary DNA-carbon-based nanomaterial solution, polishing glassy carbon electrode with aluminum oxide powder, immersing in chloroauric acid solution to electrodeposit gold particles, modifying with lead ion aptamer solution adding mercaptohexanol solution, adding the cDNA-carbon-based nanomaterial solution, obtaining sensor, adding the sensor to lead ion standard solutions, obtaining working electrode, using saturated silver/silver chloride electrode as reference electrode and platinum wire electrode as counter electrode, testing three-electrode system, establishing standard linear curve, placing the sensor in the solution to be tested of lead, obtaining working electrode, testing three-electrode system, detecting electrochemiluminescence signal, substituting into the standard linear curve, and obtaining concentration of lead ion in the solution to be tested. USE - Sensitivity-controllable lead ion-induced double-amplified electrochemiluminescence detection for biological sensing detection, and detecting and quantifying lead ion in soil. ADVANTAGE - The method detects lead ion with high sensitivity and excellent selectivity. Due to the introduction of the aptamer of the specific recognition element lead ion, the selectivity of the double-amplified electrochemiluminescence detection is improved, the interference of other ions coexisting with lead ion is reduced, and the specific analysis of lead ion is realized. DETAILED DESCRIPTION - Sensitivity-controllable lead ion-induced double-amplified electrochemiluminescence detection involves (1) mixing 1-(3-dimethylpropyl)-3-ethylcarbodiimide hydrochloric acid and N,N-dimethylformamide to obtain mixed solution (A), adding to carbon-based nanomaterial solution, stirring to activate the carboxyl groups on the surface of the carbon-based nanomaterial, adding amino functionalized complementary-DNA (cDNA) solution, stirring, preparing cDNA-carbon-based nanomaterial solution, (2) polishing glassy carbon electrode with aluminum oxide powder, ultrasonically processing in ethanol and water sequentially, obtaining pretreated electrode by drying in air, (3) immersing the pretreated electrode in chloroauric acid solution to electrodeposit gold particles, obtaining treated electrode, (4) modifying the lead ion aptamer solution on the treated electrode, reacting, obtaining electrode, (5) adding mercaptohexanol solution in drops on the electrode to block non-specific binding sites, heat-preserving at room temperature to obtain electrode, (6) adding the cDNA-carbon-based nanomaterial solution in drops on the electrode, reacting, obtaining sensor, (7) adding the sensor to lead ion standard solutions of different concentrations and binding, rinsing the sensor with ultrapure water and air-drying at room temperature, obtaining electrode material as working electrode, using saturated silver/silver chloride electrode as reference electrode and platinum wire electrode as counter electrode, testing three-electrode system in buffer solution containing tris(bipyridine)ruthenium(II) and tripropylamine, recording electrochemiluminescence signal and detecting by MPI-EII electrochemiluminescence analyzer, establishing a standard linear curve of the corresponding relationship between the concentration of lead ion solution and the electrochemiluminescence signal, (8) placing the sensor obtained in step (6) in the solution to be tested of lead, rinsing with ultrapure water, and naturally drying at room temperature, obtaining working electrode, using saturated silver/silver chloride as reference electrode and platinum wire electrode as counter electrode, testing three-electrode system in buffer solution containing tris(bipyridine)ruthenium(II) and tripropylamine, detecting electrochemiluminescence signal, substituting into the standard linear curve established in step (7), obtaining concentration of lead ion in the solution to be tested, and achieving highly sensitive detection of lead ion.