▎ 摘　　要

NOVELTY - Method for optical detection of insulin involves uniformly mixing the insulin standard sample solution with the graphene oxide/nano-gold complex material solution, uniformly mixing the graphene oxide/nano-gold complex material solution with water, as a control group, detecting the visible light absorbance, taking the absorbance peak value of the insulin standard sample solution compared with the shift average value of the absorbance peak value of the control group, as ordinate and the concentration of the insulin standard sample solution, as abscissa to draw a standard concentration-dependent curve, mixing the commercial insulin solution to be tested with the graphene oxide/nano-gold complex material solution, detecting the visible light absorbance and substituting the shift average into the corresponding commercial insulin calibration curve to obtain the corresponding concentration. USE - The method is useful for optical detection of insulin. ADVANTAGE - The method can rapidly detect different concentrations and different types of insulin, is simple and has high success rate and good detection stability. DETAILED DESCRIPTION - Method for optical detection of insulin involves (a) formulating different commercial insulins into several groups of insulin standard sample solutions having concentration range of 5-20 μmol/l, uniformly mixing the insulin standard sample solution with the graphene oxide/nano-gold complex material solution respectively, detecting the visible light absorbance, uniformly mixing the graphene oxide/nano-gold complex material solution with water, as a control group and detecting the visible light absorbance, (b) taking the absorbance peak value of the insulin standard sample solution compared with the shift average value of the absorbance peak value of the control group, as ordinate and the concentration of the insulin standard sample solution, as abscissa to draw a standard concentration-dependent curve for subsequent insulin detection, (c) mixing the commercial insulin solution to be tested with the graphene oxide/nano-gold complex material solution, detecting the visible light absorbance to obtain the shift average value of the absorbance peak value of the solution compared with the absorbance peak value of the control group and substituting the shift average into the corresponding commercial insulin calibration curve obtained in step (b) to obtain the corresponding concentration.