▎ 摘　　要

NOVELTY - The slide or coverslip has a substrate and a graphene oxide layer provided over at least a portion of the substrate. The graphene oxide layer has a predetermined average thickness, suitably less than 2 nanometers. The slide or coverslip also includes spacer layer comprises a polymeric layer, suitably the polymeric layer is formed from a biocompatible (co)polymer, suitably a polymer that substantially does not alter the growth, such as numbers or size or the morphology, of the target sample. The spacer layer comprises a polymeric layer formed from (co)polymers selected from one or more of polystyrene, polymethyl(meth)acrylate, polyurethane, polycarbonate, chitosan, polyethyleneimine, polyvinyl chloride, polydimethylsiloxane, polyamideimide, polydimethylsiloxane, polyethersulphone, acrylonitrile butadiene styrene and polylysine. USE - Slide or coverslip for use with fluorescence microscope. Can be used in near-field fluorescence microscopy, selective plane illumination microscopy (SPIM), super-resolution fluorescence microscopy, stochastic optical reconstruction microscopy (STORM), diffraction limited fluorescence imaging, stimulated emission depletion microscopy (STED), structured illumination microscopy and confocal microscopy. ADVANTAGE - Ensures that the optical signal from the fluorophores can propagate through the polymer layer can be detected in the optical microscope. Reduces image blur due to their motion since positive charge encourages the strong binding with biological cells. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method for increasing the contrast of a fluorescent sample in fluorescence microscopy; and (2) a use of slide or coverslip. DESCRIPTION OF DRAWING(S) - The drawing shows the image of STORM that can be used to study the self-assembly of aggregates of surfactant peptide.