• 专利标题:   Method for preparing electrode that is utilized for detecting dopamine, involves dispersing reduced graphene oxide in anhydrous ethanol, removing reduced graphene oxide, followed by activating screen print electrode and scanning.
  • 专利号:   CN103913495-A, CN103913495-B
  • 发明人:   BAI X, CHU K, GU L, HUA Z, QIN Q, CHENG H, XU L
  • 专利权人:   UNIV HOHAI
  • 国际专利分类:   G01N027/327
  • 专利详细信息:   CN103913495-A 09 Jul 2014 G01N-027/327 201465 Pages: 12 Chinese
  • 申请详细信息:   CN103913495-A CN10007432 09 Jan 2013
  • 优先权号:   CN10007432

▎ 摘  要

NOVELTY - An electrode preparing method involves dispersing reduced graphene oxide (rGO) in anhydrous ethanol ultrasonically to obtain rGO suspension at concentration of 100-2000 mg/l, removing the rGO suspension, and coating dropwise with the amount of 10-30 mu l after activation treatment of screen print electrode (SPE) to obtain SPE/rGO followed by dissolving 0.005-0.025 g beta -cyclodextrin ( beta -CD) and 500-5000 U glucose oxidase (GOx) in each 1 ml of phosphate buffered saline at pH of 6.2-8.0 and scanning the mix solution of SPE/rGO to obtain a SPE/rGO/ beta -CD/GOx electrode. USE - Method for preparing an electrode that is utilized for detecting dopamine. ADVANTAGE - The method enables preparing the electrode with high sensitivity and better selectivity in a simple, rapid and cost-effective manner. DETAILED DESCRIPTION - An electrode preparing method involves dispersing reduced graphene oxide (rGO) in anhydrous ethanol ultrasonically for 0.5-8 hours to obtain rGO suspension at concentration of 100-2000 mg/l, removing the rGO suspension, and coating dropwise with the amount of 10-30 mu l after activation treatment of screen print electrode (SPE) to obtain SPE/rGO followed by dissolving 0.005-0.025 g beta -cyclodextrin ( beta -CD) and 500-5000 U glucose oxidase (GOx) in each 1 ml of phosphate buffered saline at a pH of 6.2-8.0 and scanning the mix solution of SPE/rGO, where the scanning potential range is 0.2-0.8 V to obtain a SPE/rGO/ beta -CD/GOx electrode.