• 专利标题:   Amphiphilic graphene quantum dot material used as nuclear target imaging fluorescent probe for Hela cell or other living cells, comprises graphene quantum dots including carbon, nitrogen, chlorine, oxygen, and hydrogen.
  • 专利号:   CN108485659-A
  • 发明人:   WANG L, LI W, LI M, LIU Y, PAN D, WU M
  • 专利权人:   UNIV SHANGHAI
  • 国际专利分类:   C09K011/65, G01N021/64, B82Y020/00, B82Y030/00
  • 专利详细信息:   CN108485659-A 04 Sep 2018 C09K-011/65 201875 Pages: 12 Chinese
  • 申请详细信息:   CN108485659-A CN10145925 12 Feb 2018
  • 优先权号:   CN10145925

▎ 摘  要

NOVELTY - An amphiphilic graphene quantum dot material comprises a graphene quantum dot comprising chlorine atoms attached to carbon atoms of graphite, or amino or hydroxyl group of nanosheet. The quantum dots have an average diameter of 1-5 nm (preferably 5 nm), and an average thickness of 0.6-2 nm (preferably 1 nm). The quantum dots comprise carbon, nitrogen, chlorine, oxygen, and hydrogen. USE - An amphiphilic graphene quantum dot material used as a nuclear target imaging fluorescent probe for Hela cell or other living cells (claimed). ADVANTAGE - The amphiphilic graphene quantum dot material is prepared using simple, environment-friendly, and inexpensive method. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparation of the amphiphilic graphene quantum dot material, comprising: (A) slowly adding 0.05-0.5 g of 1,5-diaminonaphthalene to 10-50 mL solvent mixture of ethanol and chloroform while ultrasonically stirring for 10-50 minutes, transferring the solution to not less than 50 mL PTFE reaction kettle, and performing hydrothermal reaction at 120-230 degrees C for 6-24 hours to obtain an amphiphilic graphene quantum dot product; (B) naturally cooling the amphiphilic graphene quantum dot product, filtering in 220 nm micropore filter membrane, transferring filtrate to a dialysis bag, carrying out dialysis, and carrying out separation and purification to obtain amphiphilic graphene quantum dot solution; and (C) carrying out conversion treatment, rotary evaporating the solvent, adding deionized water, and ultrasonically stirring for 10-50 minutes to dissolve the quantum dots in water.