▎ 摘　　要

NOVELTY - Preparing electrochemical luminous immune sensor (ECLIs) involves taking glassy carbon electrode having 4mm diameter, crushing aluminum oxide to obtain polishing powder and cleaning with ultra-pure water. The oxidation graphene/carboxylated carbon nano tube loading gold-adulterated cerium dioxide nano particle (GO/MWCNTs-COOH/Au-CeO2) chitosan solution (6 mu l of 2-14mg/ml) is added drop-wise to the electrode surface, and drying at room temperature. The tumor marker antibodies (6 mu l of 6-12mg/ml) are dropped. The electrode surface is washed with ultra-pure water, and then stored at 4 degrees C. USE - Method for preparing electrochemical luminous immune sensor for detecting multiple tumor markers (claimed). ADVANTAGE - The method enables to prepare electrochemical luminous immune sensor for early diagnosis of tumor. DETAILED DESCRIPTION - Preparing electrochemical luminous immune sensor (ECLIs) involves taking glassy carbon electrode having 4mm diameter, crushing aluminum oxide to obtain polishing powder and cleaning with ultra-pure water. The oxidation graphene/carboxylated carbon nano tube loading gold-adulterated cerium dioxide nano particle (GO/MWCNTs-COOH/Au-CeO2) chitosan solution (6 mu l of 2-14mg/ml) is added drop-wise to the electrode surface, and drying at room temperature. The tumor marker antibodies (6 mu l of 6-12mg/ml) are dropped. The electrode surface is washed with ultra-pure water, and then stored at 4 degrees C. The active surface of the non-specific sites of electrode are closed using 3 mu l of 1-2 wt.% bovine serum albumin (BSA) solution. The electrode surface is washed with ultra-pure water, and then stored at 4 degrees C. The tumor marker antigen solution (6 mu l of 0.05-100ng/ml) is added dropwise, and then stored at 4 degrees C to obtain an electrochemical immunoassay sensor. An INDEPENDENT CLAIM is included for a method for detecting multiple tumor markers, which involves: (A) preparing three-electrode electrochemical workstation system by taking Ag/AgCl electrode as the reference electrode, and platinum wire electrode is an auxiliary electrode in 10ml solution of concentration 50 mmol/l and 7.5-8.5 pH comprising 0.1mol/l potassium chloride and 20-140mmol/l potassium persulfate in phosphate buffered saline; (B) carrying out analyte detection using MPI-F flow injection chemistry lighting analyzer in solution comprising 0.1mol/l potassium chloride and 120mmol/l potassium persulfate in phosphate buffered saline; (C) scanning at -2V to 0V using photomultiplier tube with 750V high voltage, at scan rate 0.1V/second; and (D) recording ECL spectrum when peak is stabilized.